MABE1106

Anti-WAPL Antibody, clone 1C7-B1

clone 1C7-B1, from mouse

• Synonym(s): WAPL, Wings apart-like protein homolog, Friend of EBNA2 protein
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 1C7-B1, monoclonal
• species reactivity: human
• technique(s): immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG2aκ
• NCBI accession no.: NP_055860
• UniProt accession no.: Q7Z5K2
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... WAPAL(23063)

Description

General description

Wings apart-like protein homolog (also known as Friend of Epstein-Barr virus nuclear protein 2) is a nuclear protein encoded by the WAPL (WAPAL) gene in human. Wapl functions as a negative regulator of cohesin–chromosome association and facilitates the progression of mitosis both during interphase and prophase. In interphase, Wapl destabilizes cohesin–chromosome interaction and thereby reduces the extent of cohesin-mediated chromosome looping. In prophase, mitotic kinases phosphorylate cohesin and its regulators. Wapl then triggers the release of phosphorylated cohesin from sister chromatids, allowing sister chromosome decatenation by topoisomerase II (Topo II). (PMID 24156811).

Immunogen

Recombinant protein corresponding to human WAPL.

Application

Anti-WAPL Antibody, clone 1C7-B1 is an antibody against WAPL for use in Western Blotting, Immunoprecipitation.

Immunoprecipitation Analysis: 1.0 mL tissue culture supernantant of this antibody immunoprecipitated WAPL in HeLa cell lysate (Professor Haruhiko Siomi, Keio University School of Medicine).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected WAPL in 10 µg of HeLa cell lysate.

Target description

~175 kDa observed

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable