MABD122A4

Anti-CD73, clone AA60-E3-3, Alexa Fluor^™ 488 conjugate Antibody

clone AA60-E3-3, from mouse, ALEXA FLUOR^™ 488

• Synonym(s): CD-73, 5′-nucleotidase, 5′-NT, ecto-5′-nucleotidase
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.44

Properties

• biological source: mouse
• Quality Level: 100
• conjugate: ALEXA FLUOR^™ 488
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: AA60-E3-3, monoclonal
• species reactivity: human
• species reactivity (predicted by homology): mouse (immunogen homology)
• technique(s): immunocytochemistry: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_001191742
• UniProt accession no.: Q61503
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... NT5E(4907)

Description

General description

CD73, also known as Ecto-5′-nucleotidase, catalyzes the conversion of purine 5′ mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation, being expressed on subsets of B-cells and T-cells. CD73 is also a marker of undifferentiated mesenchymal stem cells and is often used to identified stem cell populations.

Immunogen

Whole Mouse Dermal Lymphatic Endothelial Cells (DLEC)

Application

Evaluated by Immunocytochemistry on human bone marrow mesenchymal stem cells. Immunocytochemical analysis: A 1:50 dilutions of this antibody detected CD73 on 1X10^6 human adipose and bone marrow mesenchymal stem cells.

Research Category
Stem Cell Research

Research Sub Category
Mesenchymal Stem Cells

This Anti-CD73, clone AA60-E3-3 is validated for use in Immunocytochemistry for the detection of CD73.

Quality

Evaluated by Immunocytochemistry on human adipose mesenchymal stem cells.
Immunocytochemical analysis: A 1:50 dilutions of this antibody detected CD73 on 1X10^6 human adipose and bone marrow mesenchymal stem cells.

Target description

The unconjugated parent antibody (Catalog No. MABD122) has an observed MW at 63 kDa

Physical form

Protein G Purified

Purified mouse monoclonal IgG1κ conjugated to Alexa Fluor^™ 488 in PBS with 0.1% sodium azide and 15mg/ml BSA.

Storage and Stability

Maintain refrigerated at 2-8ºC protected from light in undiluted aliquots for up to 6 months from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable