MABC528
Anti-SSTR5/SS5R (P335L mutation) Antibody, clone D2F2
clone D2F2, from mouse
Synonym(s):
Somatostatin receptor type 5, SS-5-R, SS5-R, SS5R
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
mouse
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
D2F2, monoclonal
species reactivity
human
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... SSTR5(6755)
General description
Somatostatin receptor subtype 5 (SSTR5) mediates the inhibitory effect of somatostatin on insulin expression/secretion and cell proliferation. A number of single nucleotide polymorphisms (SNPs) of SSTR5 have been identified, including P335L, a nonsynonymous SNP located in the protein C-terminal region and encrypted by the codon CCG (proline) or the codon CTG.
SSTR5 SNP P335L widely exists in the human population, in patients with pancreatic cancer, and is race-dependent. SSTR5 SNP P335 overexpression resulted in cellular proliferation and PDX-1 overexpression in human pancreatic cancer cells. Its overexpression also blocked the inhibitory effect of SSTR5-specific analogs on human pancreatic cancer cell proliferation and on glucose-stimulated insulin secretion from mouse insulinoma cells. SSTR5 P335L is a hypofunctional protein with a potentially harmful effect on function, as well as potential latent effect, and therefore it could affect the clinical response to somatostatin analog therapy for patients with pancreatic cancer.
SSTR5 SNP P335L widely exists in the human population, in patients with pancreatic cancer, and is race-dependent. SSTR5 SNP P335 overexpression resulted in cellular proliferation and PDX-1 overexpression in human pancreatic cancer cells. Its overexpression also blocked the inhibitory effect of SSTR5-specific analogs on human pancreatic cancer cell proliferation and on glucose-stimulated insulin secretion from mouse insulinoma cells. SSTR5 P335L is a hypofunctional protein with a potentially harmful effect on function, as well as potential latent effect, and therefore it could affect the clinical response to somatostatin analog therapy for patients with pancreatic cancer.
Immunogen
Linear peptide corresponding to human SSTR5/SS5R (P335L mutation).
Application
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected SSTR5/SS5R (P335L mutation) in normal human pancreas and human pancreatic cancer tissues.
Western Blotting Analysis: A representative lot from an independent laboratory detected SSTR5/SS5R (P335L mutation) in HA-SSTR5 (P335L mutation) transfected HEK293 cell lysate (Zhou, G., et al. (2011). World J Surg. 35(8):1715-1724.).
Immunocytochemistry Analysis: A representative lot from an independent laboratory detected SSTR5/SS5R (P335L mutation) in Panc-1 cells expressing SSTR5 (P335L mutation), and not in Panc-1 cells or CAPAN-1 cells expressing wild type SSTR5 (Zhou, G., et al. (2011). Surgery. 150(6):1136-1142.).
Western Blotting Analysis: A representative lot from an independent laboratory detected SSTR5/SS5R (P335L mutation) in CAPAN-1 and Panc-1 cell lysates expressing SSTR5/SS5R (P335L mutation) (Zhou, G., et al. (2011). Surgery. 150(6):1136-1142.).
Western Blotting Analysis: A representative lot from an independent laboratory detected SSTR5/SS5R (P335L mutation) in HA-SSTR5 (P335L mutation) transfected HEK293 cell lysate (Zhou, G., et al. (2011). World J Surg. 35(8):1715-1724.).
Immunocytochemistry Analysis: A representative lot from an independent laboratory detected SSTR5/SS5R (P335L mutation) in Panc-1 cells expressing SSTR5 (P335L mutation), and not in Panc-1 cells or CAPAN-1 cells expressing wild type SSTR5 (Zhou, G., et al. (2011). Surgery. 150(6):1136-1142.).
Western Blotting Analysis: A representative lot from an independent laboratory detected SSTR5/SS5R (P335L mutation) in CAPAN-1 and Panc-1 cell lysates expressing SSTR5/SS5R (P335L mutation) (Zhou, G., et al. (2011). Surgery. 150(6):1136-1142.).
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Apoptosis - Additional
This Anti-SSTR5/SS5R (P335L mutation) Antibody, clone D2F2 is validated for use in western blotting, ICC & IHC for the detection of SSTR5/SS5R (P335L mutation).
Quality
Evaluated by Western Blotting in Panc-1 cell lysate and Mia Paca-2 cell lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected SSTR5/SS5R (P335L mutation) in 10 µg of Panc-1 cell lysate and not in Mia Paca-2 cell lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected SSTR5/SS5R (P335L mutation) in 10 µg of Panc-1 cell lysate and not in Mia Paca-2 cell lysate.
Target description
~39 kDa observed
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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