MABC525
Anti-BAG-1 Antibody, clone 3.10G3E2
clone 3.10G3E2, from mouse
Synonym(s):
BAG family molecular chaperone regulator 1, BAG-1, Bcl-2-associated athanogene 1
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
mouse
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
3.10G3E2, monoclonal
species reactivity
human
technique(s)
ELISA: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... BAG1(573)
General description
BAG family molecular chaperone regulator 1 (BAG-1), also known as Bcl-2-associated athanogene 1, blocks a step in a pathway leading to apoptosis or programmed cell death. BAG-1 enhances the anti-apoptotic effects of BCL2 representing a link between growth factor receptors and anti-apoptotic mechanisms. BAG-1 is ubiquitously expressed throughout most tissues, except the liver, colon, breast, and uterine myometrium.
Specificity
This antibody detects 3 observed isoforms: BAG-1(S) at ~38 kDa, BAG-1 (M) at ~45 kDa & BAG-1 (L) at ~52 kDa.
Immunogen
GST-tagged recombinant protein corresponding to Human BAG-1.
Application
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Apoptosis - Additional
This Anti-BAG-1 Antibody, clone 3.10G3E2 is validated for use in Western Blotting and Immunohistochemistry and Immunoprecipitation and ELISA for the detection of BAG-1.
Western Blot Analysis: A representative lot of this antibody detected BAG-1 in MCF-7 cell lysate (Brimmell, M., et al., (1999). British Journal of Cancer 81(5):1042–1051).
Western Blot Analysis: A representative lot of this antibody detected BAG-1 in colonic epithelial cell lysate (Arhel, N. J., et al., (2003). Int. J. Cancer: 106, 364–371).
Western Blot Analysis: A representative lot of this antibody detected BAG-1(L, M, S) in BAG-1(L, M, S) overexpressed MCF-7 cell lysates.
Immunohistochemistry Analysis: A representative lot of this antibody detected BAG-1 in breast cancer tissue sections (Cutress, R. I., et al., (2003). Oncogene. 22:4973–4982).
Immunohistochemistry Analysis: A representative lot of this antibody detected BAG-1 in breast cancer tissue sections.
Immunoprecipitation Analysis: A representative lot of this antibody immunoprecipitated BAG-1 from endogenouse MCF-7 cell lysates.
Immunoprecipitation Analysis: A representative lot of this antibody immunoprecipitated BAG-1 from Carcinoma-derived HT29 and transformed adenoma PC/AA/C1/SB10 cell lysates (Arhel, N. J., et al., (2003). Int. J. Cancer: 106, 364–371).
Interaction Assay/ELISA: A representative lot of this antibody detected BAG-1 by a BAG-1:HSC70 interaction/ELISA assay (Sharp, A., et al., (2009). FEBS Letters 583:3405–3411).
Western Blot Analysis: A representative lot of this antibody detected BAG-1 in colonic epithelial cell lysate (Arhel, N. J., et al., (2003). Int. J. Cancer: 106, 364–371).
Western Blot Analysis: A representative lot of this antibody detected BAG-1(L, M, S) in BAG-1(L, M, S) overexpressed MCF-7 cell lysates.
Immunohistochemistry Analysis: A representative lot of this antibody detected BAG-1 in breast cancer tissue sections (Cutress, R. I., et al., (2003). Oncogene. 22:4973–4982).
Immunohistochemistry Analysis: A representative lot of this antibody detected BAG-1 in breast cancer tissue sections.
Immunoprecipitation Analysis: A representative lot of this antibody immunoprecipitated BAG-1 from endogenouse MCF-7 cell lysates.
Immunoprecipitation Analysis: A representative lot of this antibody immunoprecipitated BAG-1 from Carcinoma-derived HT29 and transformed adenoma PC/AA/C1/SB10 cell lysates (Arhel, N. J., et al., (2003). Int. J. Cancer: 106, 364–371).
Interaction Assay/ELISA: A representative lot of this antibody detected BAG-1 by a BAG-1:HSC70 interaction/ELISA assay (Sharp, A., et al., (2009). FEBS Letters 583:3405–3411).
Quality
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected BAG-1 in 10 µg of HeLa cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected BAG-1 in 10 µg of HeLa cell lysate.
Target description
~38, 45, & 52 kDa observed.
This antibody detects 3 observed isoforms at ~38 kDa (S), ~45 kDa (M) & ~52 kDa (L).
This antibody detects 3 observed isoforms at ~38 kDa (S), ~45 kDa (M) & ~52 kDa (L).
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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