MABC1168
Anti-LIMD2 Antibody, clone 18.30
clone 18.30, from mouse
Synonym(s):
LIM domain-containing protein
Sign Into View Organizational & Contract Pricing
All Photos(2)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
18.30, monoclonal
species reactivity
human
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... LIMD2(80774)
General description
LIM domain-containing protein 2 (UniProt Q9BT23) is encoded by the LIMD2 (also known as SB143) gene (Gene ID 80774) in human. The LIM domain is organized as a tandem zinc-finger structure that functions as a modular protein-binding interface and LIM domain-containing proteins play diverse roles in gene expression, cyto-architecture, cell adhesion, cell motility, and signal transduction. Human LIM domain-only (LMO) proteins are involved in many normal developmental processes, as well as in the onset and progression of several cancers, including T-cell leukemia, breast cancer, and neuroblastoma. LIMD2 is a LMO protein that is highly expressed in papillary thyroid carcinoma (PTC) lymph node metastasis (LNM), but absent from the primary tumor or normal thyroid tissue in matched PTC patient samples. LIMD2 regulates cell motility and affects tumor progression via its role in the integrin-linked kinase (ILK) pathway. LIMD2 is reported to activate ILK activity via direct binding to ILK kinase domain near the active site, LIMD2-knockdown in cancer cells results in reduced cell migration. Likewise, epitopic LIMD2 expression promotes the migration of ILK-expressing fibroblasts, but not fibroblasts lacking ILK.
Specificity
Clone 18.30 detected endogenous LIMD2 in human (HEK293, U2OS, and TPC-1) cell lysates, as well as recombinant human LIMD2, but not LIMD1 or AJUBA protein (Peng, H., et al. (2014). Cancer Res. 74(5):1390-1403).
Immunogen
GST-tagged recombinant full-length human LIMD2.
Application
Anti-LIMD2 Antibody, clone 18.30 is an antibody against LIMD2 for use in Western Blotting, Immunohistochemistry (Paraffin), Immunocytochemistry.
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Adhesion (CAMs)
Adhesion (CAMs)
Western Blotting Analysis: 0.25 µg/mL from a representative lot detected LIMD2 in human U2OS osteosarcoma cell lysate.
Immunocytochemistry Analysis: A representative lot detected exogenously expressed LIMD2 protein constructs in formaldehyde-fixed, Triton X-100-permeabilized cells transfected with epitopically tagged LIMD2 (Peng, H., et al. (2014). Cancer Res. 74(5):1390-1403).
Immunohistochemistry Analysis: A representative lot detected LIMD2 immunoreactivity in formalin-fixed, paraffin-embedded papillary thyroid cancer and bladder tumor sections (Peng, H., et al. (2014). Cancer Res. 74(5):1390-1403).
Western Blotting Analysis: A representative lot detected endogenous LIMD2 in HEK293 and U2OS cell lysates, as well as epitopically tagged LIMD2, but not LIMD1 or AJUBA recombinant protein constructs (Peng, H., et al. (2014). Cancer Res. 74(5):1390-1403).
Immunocytochemistry Analysis: A representative lot detected exogenously expressed LIMD2 protein constructs in formaldehyde-fixed, Triton X-100-permeabilized cells transfected with epitopically tagged LIMD2 (Peng, H., et al. (2014). Cancer Res. 74(5):1390-1403).
Immunohistochemistry Analysis: A representative lot detected LIMD2 immunoreactivity in formalin-fixed, paraffin-embedded papillary thyroid cancer and bladder tumor sections (Peng, H., et al. (2014). Cancer Res. 74(5):1390-1403).
Western Blotting Analysis: A representative lot detected endogenous LIMD2 in HEK293 and U2OS cell lysates, as well as epitopically tagged LIMD2, but not LIMD1 or AJUBA recombinant protein constructs (Peng, H., et al. (2014). Cancer Res. 74(5):1390-1403).
Quality
Evaluated by Western Blotting in HEK293 cell lysate.
Western Blotting Analysis: 0.25 µg/mL of this antibody detected LIMD2 in human HEK293 kidney embryonic cell lysate.
Western Blotting Analysis: 0.25 µg/mL of this antibody detected LIMD2 in human HEK293 kidney embryonic cell lysate.
Target description
~17 kDa observed. 14.07 kDa calculated. Uncharacterized band(s) may appear in some lysates.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service
