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MAB8152

Sigma-Aldrich

Anti-West Nile Virus/Kunjin Antibody, NS1, clone 3.1112G

ascites fluid, clone 3.1112G, Chemicon®

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

antibody form

ascites fluid

antibody product type

primary antibodies

clone

3.1112G, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgM

shipped in

wet ice

Specificity

Specific for the non-structural protein 1 of West Nile/Kunjin virus. Kunjin (KUN) is very closely related to some strains of West Nile virus, and has been classified as a subtype of West Nile.

Immunogen

Epitope: NS1

Application

Immunofluorescence: 1:2 to 1:50

EIA 1:20 to 1:200

Western Blot 1:20 to 1:200

Immunohistochemistry

Optimal working dilutions must be determined by the end user.
Research Category
Infectious Diseases
Research Sub Category
Infectious Diseases - Viral
This Anti-West Nile Virus/Kunjin Antibody, NS1, clone 3.1112G is validated for use in ELISA, IF, IH, WB for the detection of West Nile Virus/Kunjin.

Physical form

Ascites fluid with 0.1% sodium azide as a preservative.
Unpurified

Storage and Stability

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
West Nile Virus positive patient sample

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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M A Loroño-Pino et al.
Clinical and vaccine immunology : CVI, 16(5), 749-755 (2009-03-27)
An epitope-blocking enzyme-linked immunosorbent assay (b-ELISA) was evaluated for the diagnosis of West Nile virus (WNV) infections in humans. Sera from patients diagnosed with WNV infections from an outbreak in 2003 in Colorado and from patients diagnosed with dengue virus
Replication of West Nile virus in equine peripheral blood mononuclear cells.
David Garcia-Tapia, Christie M Loiacono, Steven B Kleiboeker
Veterinary Immunology and Immunopathology null
Jeremy P Ledermann et al.
Clinical and vaccine immunology : CVI, 18(4), 580-587 (2011-02-25)
Primary West Nile virus (WNV) infections can be diagnosed using a number of tests that detect infectious particles, nucleic acid, and specific IgM and/or IgG antibodies. However, serological identification of the infecting agent in secondary or subsequent flavivirus infections is
Multiple amino acid changes at the first glycosylation motif in NS1 protein of West Nile virus are necessary for complete attenuation for mouse neuroinvasiveness.
Melissa C Whiteman,Jason A Wicker,Richard M Kinney,Claire Y-H Huang,Tom Solomon,Alan D T Barrett
Vaccine null
Carlos Machain-Williams et al.
Journal of wildlife diseases, 49(3), 690-693 (2013-06-20)
Surveillance for evidence of West Nile virus (WNV) infection in Morelet's crocodiles (Crocodylus moreletii) was conducted in Campeche State, Mexico, in 2007. Sera from 62 crocodiles (32 free-ranging and 30 captive) were assayed for antibodies to WNV by epitope-blocking enzyme-linked

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