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MAB5514

Sigma-Aldrich

Anti-Choline Transporter Antibody, clone 62-2E8

ascites fluid, clone 62-2E8, Chemicon®

Synonym(s):

CHT

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

antibody form

ascites fluid

antibody product type

primary antibodies

clone

62-2E8, monoclonal

species reactivity

human, mouse

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

NM_021815.2

UniProt accession no.

Q9GZV3

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... SLC5A7(60482)

Specificity

Hemicholinium-3-sensitive choline transporter (CHT).

Immunogen

Recombinant protein from the C-terminus of human CHT.

Application

Detect Choline Transporter using this Anti-Choline Transporter Antibody, clone 62-2E8 validated for use in IC, IH & WB.
Research Category
Neuroscience
Research Sub Category
Ion Channels & Transporters
Western blot. The antibody detects a 65 kDa band in mouse brain tissue lysate. The CHT immunoreactivity was most abundant in the striatum.

Immunohistochemistry 1:2,000 - 1:8,000

Immunocytochemistry

Optimal working dilutions must be determined by end user.

Physical form

Ascites fluid. Liquid. Contains no preservative.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Anthony C Wong et al.
Journal of virology, 84(1), 163-175 (2009-10-23)
Ebolavirus (EBOV) entry into cells requires proteolytic disassembly of the viral glycoprotein, GP. This proteolytic processing, unusually extensive for an enveloped virus entry protein, is mediated by cysteine cathepsins, a family of endosomal/lysosomal proteases. Previous work has shown that cleavage
Vinay Parikh et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 33(6), 2326-2337 (2013-02-09)
Functional variation in the gene encoding the presynaptic choline transporter (CHT) has been linked to attention-deficit/hyperactivity disorder. Here, we report that a heterozygous deletion in the CHT gene in mice (CHT(+/-)) limits the capacity of cholinergic neurons to sustain acetylcholine
A V Terry et al.
Neuroscience, 146(3), 1316-1332 (2007-04-17)
First and second generation antipsychotics (FGAs and SGAs) ameliorate psychotic symptoms of schizophrenia, however, their chronic effects on information processing and memory function (i.e. key determinants of long term functional outcome) are largely unknown. In this rodent study the effects

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