MAB5386X

Anti-Nerve Growth Factor Receptor Antibody, p75, Alexa Fluor^™ 488

clone ME20.4, Chemicon^®, from mouse

• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• conjugate: ALEXA FLUOR^™ 488
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: ME20.4, monoclonal
• species reactivity: rabbit, human, pig, sheep
• should not react with: mouse, chicken, rat
• manufacturer/tradename: Chemicon^®
• technique(s): flow cytometry: suitable; immunocytochemistry: suitable
• isotype: IgG1
• NCBI accession no.: NM_002507.2
• UniProt accession no.: P08138
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... NGFR(4804)

Description

General description

The low affinity NGFR (Nerve growth factor receptor) is a 75kDa membrane-spanning glycoprotein lacking intrinsic tyrosine kinase activity. p75NGFR interacts with TrkA, the high affinity NGF receptor and potentiates TrkA signaling at low NGF concentrations. The p75 receptor binds nerve growth factor, brain-derived neurotrophic factor, neurotrophin-3 and neurotrophin-4 with varying specificities. The p75NGFR plays an important role in neurotrophic factor signaling and has been shown to modulate the susceptibility of selective cellular populations to programmed cell death. It is expressed on many neuronal cells types including many embryonic forms and the receptor can be used to isolate neuronal progenitor cells

Specificity

Reacts with the low affinity nerve growth factor receptor (NGFR p75). The epitope recognized by the antibody maps within the third or fourth cysteine-rich repeats of the NGFR molecule. Reactivity has not been observed with rat, chicken, or mouse NGFR.

Immunogen

Human melanoma cell line.

Application

This Anti-Nerve Growth Factor Receptor Antibody, p75, Alexa Fluor 488 is validated for use in FC, IC for the detection of Nerve Growth Factor Receptor.

Storage and Stability

Aliquot and store at -20 ºC, or glycerol (ASC grade or better) can be added (1:1) and the antibody stored at -20ºC for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable