MAB4056

Anti-Angiotensin Converting Enzyme Antibody, clone i2H5

clone i2H5, Chemicon^®, from mouse

• Synonym(s): ACE, CD143, DCP1, Peptidyl-dipeptidase A, Peptidase P
• UNSPSC Code: 12352203
• eCl@ss: 32160702

Properties

• biological source: mouse
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: i2H5, monoclonal
• species reactivity: human
• manufacturer/tradename: Chemicon^®
• technique(s): ELISA: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable
• isotype: IgG1
• suitability: not suitable for immunohistochemistry (Paraffin)
• NCBI accession no.: NM_000789.2; NM_152830.1; NM_152831.1
• UniProt accession no.: P12821
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... AGT(183)

Description

General description

The previously assigned protein identifier P22966 has been merged into P12821. Full details can be found on the UniProt database.

Specificity

Angiotensin-converting enzyme [ACE] (N-terminal)

Immunogen

Angiotensin-converting enzyme from human lung

Application

Anti-Angiotensin Converting Enzyme Antibody, clone i2H5 is an antibody against Angiotensin Converting Enzyme for use in ELISA, IP, IC, IH.

Immunohistochemistry (Danilov et al., 1987; Falkenhahn et al., 1995; Morrell et al., 1995) : 1:1,000 on fresh frozen or 4% paraformaldehyde fixed tissues. Does not work on paraffin embedded tissue.

Immunocytochemistry: 1:100. Reacts with endothelial cells, macrophages (activated) and THP1 cells (monocyte line).

Immunoprecipitation: 1:1,000

ELISA (Stevens et al., 1990): 1:1,000

Structure-function of ACE (Danilov et al., 1994)

Optimal working dilutions must be determined by the end user.

Research Category
Neuroscience

Research Sub Category
Hormones & Receptors

Physical form

Format: Purified

Purified immunoglobulin. Liquid in 100 mM borate buffer, pH 8.0 and 150 mM NaCl.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H360FD
• pcodes: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Hazard Classifications: Repr. 1B
• Storage Class: 6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
• wgk_germany: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable