MAB374-AF647
Anti-GAPDH Antibody, clone 6C5, Alexa Fluor™ 647 conjugate
clone 6C5, from mouse, ALEXA FLUOR™ 647
Synonym(s):
Glyceraldehyde-3-phosphate dehydrogenase, GAPDH, Peptidyl-cysteine S-nitrosylase GAPDH, GAPDH
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All Photos(2)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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biological source
mouse
conjugate
ALEXA FLUOR™ 647
antibody form
purified antibody
antibody product type
primary antibodies
clone
6C5, monoclonal
species reactivity
mouse, human
species reactivity (predicted by homology)
cat (based on 100% sequence homology), fish (based on 100% sequence homology), porcine (based on 100% sequence homology), rat (based on 100% sequence homology), rabbit (based on 100% sequence homology), canine (based on 100% sequence homology)
technique(s)
immunocytochemistry: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... GAPDH(2597)
mouse ... Gapdh(14433)
rabbit ... Gapdh(100009074)
General description
Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is an ubiquitous glycolytic enzyme present in reasonably high levels in almost all tissues. The complete and functional enzyme is a tetramer with each of four identical subunits occupying the vertex of a tetrahedron. Its binding domains also include one principally interacting with NAD+ and another interacting with glyceraldehyde 3-phosphate (GAP). As a ′house-keeping′ enzyme, it catalyzes the synthesis of 1,3-biphosphoglycerate, a "high energy" intermediate used for the synthesis of ATP. Besides its cytoplasmic role in metabolism, it is also involved in the initial stages of apoptosis or oxidative stress response where it is translocated to the nucleus. Such actions may reflect the role of GAPDH in DNA repair or as one nuclear carrier for apoptotic molecules. GAPDH can also bind specifically to proteins implicated in the pathogenesis of a variety of neurodegenerative disorders, including the beta-amyloid precursor protein and the Huntingtin protein where reduced function of GAPDH is associated with Alzheimer′s and Huntington′s disease fibroblasts. GAPDH has also been identified as a potential target for nitric oxide (NO)-mediated cellular toxicity.
Specificity
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from skeletal muscle. Antibody also recognizes cardiac GAPDH. GAPDH enzyme is detected in many non-muscle cells lines including HeLa, HCT-116 cells, U937 and THP-1 cells among others.
It has been reported that this antibody does not react with GAPDH from E. coli.
Immunogen
Glyceraldehyde-3-phosphate dehydrogenase from rabbit muscle.
Application
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected GAPDH in NIH/3T3 cells.
The unconjugated version of this antibody (Cat. No. MAB374) has been demonstrated to perform in WB, ELISA, ICC, IHC, and IP.
The unconjugated version of this antibody (Cat. No. MAB374) has been demonstrated to perform in WB, ELISA, ICC, IHC, and IP.
Research Category
Signaling
Signaling
Research Sub Category
Signaling Neuroscience
Signaling Neuroscience
This Anti-GAPDH Antibody, clone 6C5, Alexa Fluor™ 647 conjugate is validated for use in Immunocytochemistry for the detection of GAPDH.
Quality
Evaluated by Immunocytochemistry in HUVEC cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected GAPDH in HUVEC cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected GAPDH in HUVEC cells.
Target description
~38 kDa observed
Physical form
Protein A purified
Purified mouse conjugate antibody in buffer containing PBS 15 mg/ml BSA and 0.1% Sodium Azide..
Storage and Stability
Stable for 1 year at room temperature from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Other Notes
Concentration: Please refer to lot specific datasheet.
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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