MAB3104
Anti-Calpain I Antibody, large subunit, clone P9
ascites fluid, clone P9, Chemicon®
Synonym(s):
mu-Calpain, μ-Calpain
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
mouse
Quality Level
antibody form
ascites fluid
antibody product type
primary antibodies
clone
P9, monoclonal
species reactivity
hamster, mouse, human, rat
should not react with
canine
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
dry ice
Gene Information
human ... CAPN1(823)
General description
Calpains are non-lysosomal cysteine proteeases which absolutely require calcium for activity. There are two major calpain isozymes: μ-calpain and m-calpain, which require micromolar or millimolar calicum for activity, respectively, in invitro assays. The major calpain isozymes are composed of two subunits: an 80kDa catalytic subunit; and a 30kDa regulatory subunit. The large and small subunits remain associated in the presence of calcium after proteolytic activation of the zymogen.
Specificity
Reacts with human calpain-I (μ-Calpain). P9 does not cross with human placental m-calpain (calpain II). This antibody will immunoprecipitate proteolytically active calpains.
Immunogen
Calpain-I large subunit from human erythrocyte calpain.
Epitope: large subunit
Application
Anti-Calpain I Antibody, large subunit, clone P9 is an antibody against Calpain I for use in ELISA, IP, WB & IC.
Immunoblot: 1:1,000 Detects an 80kDa large subunit μ-calpain, and will also detect the subunit in the non-activated proform of the zymogen, showing a band of ~110kDa. WI-38 human fibroblasts, or human erthryocytes are suggested as positive controls. Immunocytochemistry: 1:100 on A431 epidermal carcinoma.
Immunoprecipitation
ELISA: 1:6,000 (against the immunogen)
Optimal working dilutions must be determined by the end user.
Immunoprecipitation
ELISA: 1:6,000 (against the immunogen)
Optimal working dilutions must be determined by the end user.
Research Category
Apoptosis & Cancer
Metabolism
Apoptosis & Cancer
Metabolism
Research Sub Category
Apoptosis - Additional
Enzymes & Biochemistry
Apoptosis - Additional
Enzymes & Biochemistry
Physical form
Liquid
Storage and Stability
Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
Analysis Note
Control
WI-38 human fibroblasts, or human erthryocytes are suggested as positive controls.
WI-38 human fibroblasts, or human erthryocytes are suggested as positive controls.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Colleen Tagliarino et al.
Cancer biology & therapy, 2(2), 141-152 (2003-05-17)
Beta-lapachone (beta-Lap) triggers apoptosis in a number of human breast and prostate cancer cell lines through a unique apoptotic pathway that is dependent upon NQO1, a two-electron reductase. Recently, our laboratory showed that beta-lap-exposed MCF-7 cells exhibited an early increase
Sohila Zadran et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 30(3), 1086-1095 (2010-01-22)
Calpain is a calcium-dependent protease that plays a significant role in synaptic plasticity, cell motility, and neurodegeneration. Two major calpain isoforms are present in brain, with mu-calpain (calpain1) requiring micromolar calcium concentrations for activation and m-calpain (calpain2) needing millimolar concentrations.
Shafi M Kuchay et al.
Molecular and cellular biology, 27(17), 6038-6052 (2007-06-20)
Calpains are ubiquitous calcium-regulated cysteine proteases that have been implicated in cytoskeletal organization, cell proliferation, apoptosis, cell motility, and hemostasis. Gene targeting was used to evaluate the physiological function of mouse calpain-1 and establish that its inactivation results in reduced
Chia-Fang Wang et al.
Molecular and cellular biology, 32(16), 3321-3332 (2012-06-20)
Long-term memory requires the activity-dependent reorganization of the synaptic proteome to modulate synaptic efficacy and consequently consolidate memory. Activity-regulated RNA translation can change the protein composition at the stimulated synapse. Cytoplasmic polyadenylation element-binding protein 3 (CPEB3) is a sequence-specific RNA-binding
Li Wan et al.
Frontiers in molecular neuroscience, 11, 287-287 (2018-09-07)
Potassium chloride co-transporter 2 (KCC2), a major chloride transporter that maintains GABAA receptor inhibition in mature mammalian neurons, is down-regulated in the hippocampus during epileptogenesis. Impaired KCC2 function accelerates or facilitates seizure onset. Calpain, with two main subtypes of m-
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