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MAB3075

Sigma-Aldrich

Anti-G Protein Giα-1 Antibody, clone R4

ascites fluid, clone R4, Chemicon®

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

ascites fluid

antibody product type

primary antibodies

clone

R4, monoclonal

species reactivity

guinea pig, bovine, mouse, rat, human

manufacturer/tradename

Chemicon®

technique(s)

western blot: suitable

isotype

IgG2b

NCBI accession no.

NM_002069.5

UniProt accession no.

P63096

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

bovine ... Gnai1(281790)
human ... GNAI1(2770)
mouse ... Gnai1(14677)
rat ... Gnai1(25686)

Specificity

Gialpha-1. Strong reactivity with rat (100%), mouse (78%), bovine (74%) and guinea pig (48%). Slight reactivity with human (31%). Specificity confirmed by Western blot versus bovine Galphat, rat Goalpha, rat Gialpha-1, Gialpha-2, rat Gialpha-3, bovine Gsalpha, and murine GoalphaA and GoalphaB.

Immunogen

Rat brain G-proteins purified by the method of Sternweis and Robishaw (Sternweis & Robishaw, 1984).

Application

Anti-G Protein Giα-1 Antibody, clone R4 detects level of G Protein Giα-1 & has been published & validated for use in WB.
Research Category
Signaling
Research Sub Category
GPCR, cAMP/cGMP & Calcium Signaling
Western blot 1:500 - 1:2000.

Labels a single protein band of 39 - 42 kDa in bovine and rat membranes.

Optimal working dilutions must be determined by end user.

Physical form

Liquid ascites, containing sodium azide as a preservative.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Journal of neurochemistry, 159(1), 156-171 (2021-07-27)
The regulation of the serotonin transporter (SERT) by guanine nucleotide-binding protein alpha (Gα) q was investigated using Gαq knockout mice. In the absence of Gαq, SERT-mediated uptake of 5-hydroxytryptamine (5HT) was enhanced in midbrain and frontal cortex synaptosomes, but only

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