MAB2635
Anti-Mena Antibody, clone A351F7D9
clone A351F7D9, from mouse
Synonym(s):
enabled homolog (Drosophila), Protein enabled homolog, Mammalian enabled
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
A351F7D9, monoclonal
species reactivity
human, mouse, rat
technique(s)
immunohistochemistry: suitable
western blot: suitable
isotype
IgG2bκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... ENAH(55740)
General description
Mammalian Enabled (Mena) is a protein belonging to ENA/VASP family, a group of proteins that are actin-associated and implicated in a variety cell processes. Specifically, Mena is involved in cell polarity, cell motility, cytoskeleton remodeling, filopodial, and lamellipodial dynamics within migrating cells. Three forms of Mena are expressed in embryonic head tissues and are identified based on molecular weight (140 kDa, 88 kDa, and 80 kDa). In adult wild-type tissues, Mena has been isolated in brain, testis, ovary, and fat in the 80 kDa form. Studies show the 140 kDa form is expressed only in adult brains while the 88 kDa form decreases to undetectable levels by E16, (Lanier, 1999). Mena contains 4 domains of interest; the Ena/VASP homologue 1 (EVH1) domain, an EVH2 domain, a proline-rich domain, and an Arg/Leu/Glu-risch region. Expression of Mena has been observed in the myoepithelia of breast, bronchial glands, parotid, and sweat glands. Mena has also been observed in the epithelium of mouse mucosal tissues: colon-rectum, endometrium, pancreas acinar ductal, placenta vascular media, and prostate fibromuscular stroma. Most breast cancer cell lines, along with lesions from primary breast tumor, demonstrate overexpression of Mena.
Specificity
The antibody recognizes Mena and Mena alternate form (+ exon).
Immunogen
Histidine-tagged recombinant protein corresponding to human Mena and an additional fragment of Mena +, comprising only the + exon
Application
Detect Mena using this Anti-Mena Antibody, clone A351F7D9 validated for use in WB, IH.
Immunohistochemistry Analysis: A previous lot was used by an independent laboratory in IH, (Kwiatkowski, 2007).
Immunocytochemistry Analysis: A previous lot was used by an independent laboratory in IC, (Dent, 2007).
Immunocytochemistry Analysis: A previous lot was used by an independent laboratory in IC, (Dent, 2007).
Research Category
Cell Structure
Signaling
Cell Structure
Signaling
Research Sub Category
Cytoskeletal Signaling
PI3K, Akt, & mTOR Signaling
Cytoskeletal Signaling
PI3K, Akt, & mTOR Signaling
Quality
Evaluated by Western Blot in rat hippocampus tissue lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected Mena in 10 µg of rat hippocampus tissue lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected Mena in 10 µg of rat hippocampus tissue lysate.
Target description
~ 80 kDa observed MW. In brain tissues, a 140 kDa MW band is also expected to be observed.
Physical form
Format: Purified
Protein G
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Rat hippocampus tissue lysate
Rat hippocampus tissue lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Frederic Li Mow Chee et al.
Nature communications, 14(1), 1602-1602 (2023-03-25)
Interactions between cells and the extracellular matrix, mediated by integrin adhesion complexes, play key roles in fundamental cellular processes, including the sensing and transduction of mechanical cues. Here, we investigate systems-level changes in the integrin adhesome in patient-derived cutaneous squamous
Francesca Di Modugno et al.
EBioMedicine, 101, 105003-105003 (2024-02-11)
Tertiary Lymphoid Structures (TLS) correlate with positive outcomes in patients with NSCLC and the efficacy of immune checkpoint blockade (ICB) in cancer. The actin regulatory protein hMENA undergoes tissue-specific splicing, producing the epithelial hMENA11a linked to favorable prognosis in early
Kristen Skruber et al.
Current biology : CB, 30(14), 2651-2664 (2020-05-30)
Cells have many types of actin structures, which must assemble from a common monomer pool. Yet, it remains poorly understood how monomers are distributed to and shared between different filament networks. Simplified model systems suggest that monomers are limited and
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