MAB2226

Anti-Piccolo Antibody, clone 4G3.3

clone 4G3.3, from mouse

• Synonym(s): presynaptic cytomatrix protein, Aczonin
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 4G3.3, monoclonal
• species reactivity: mouse
• species reactivity (predicted by homology): human (based on 82% homology), rat (92% homology)
• technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• isotype: IgMκ
• NCBI accession no.: NP_001104266
• UniProt accession no.: Q9Y6V0
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... PCLO(27445) ; mouse ... Pclo(26875)

Description

General description

Piccolo (presynaptic cytomatrix protein or PCLO) may act as a scaffolding protein involved in the organization of synaptic active zones. Synaptic vesicles dock and fuse in the active zone of the plasma membrane at chemical synapses. The presynaptic cytoskeletal matrix (PCM), which is associated with the active zone and is situated between synaptic vesicles, is thought to be involved in maintaining the neurotransmitter release site in register with the postsynaptic reception apparatus. The cycling of synaptic vesicles is a multistep process involving a number of proteins. Among the components of the PCM that orchestrate these events are Bassoon (BSN), RIM (RBBP8), Oboe, and Piccolo (PCLO) [supplied by ENTREZGENE].

Specificity

This antibody recognizes piccolo.

Immunogen

GST-tagged recombinant protein

Application

Immunohistochemistry (Paraffin):
Representative images from a previous lot.
Piccolo staining pattern/morphology on mouse brain tissue/cerebellum. Tissue was pretreated with citrate pH 6.0, antigen retrieval. A previous lot of the antibody was diluted to 1:300, using IHC-Select Detection with HRP-DAB. Immunoreactivity is localized to the synaptic junction of neurons in the granular layer and Purkinje cells(arrow).

Research Category
Neuroscience

Research Sub Category
Synapse & Synaptic Biology

This Anti-Piccolo Antibody, clone 4G3.3 is validated for use in WB, IH(P) for the detection of Piccolo.

Quality

Routinely evaluated by western blot on rat brain lysate.

Target description

547 kDa

Physical form

Format: Purified

Purified mouse monoclonal IgMκ in buffer containing PBS and 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Rat brain lysate, mouse cerebellum

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable