Immunohistochemistry: 0.5 μg/mL on acetone-fixed cryostat sections.
Flow cytometry: 1 μg per 10E6 cells (HUVEC)
Function studies: B-N8 can be used in studies of cell adhesion molecules.
ELISA
Optimal working dilutions must be determined by the end user.
Research Category Cell Structure
Research Sub Category Adhesion (CAMs)
This Anti-VCAM-1 Antibody, clone B-N8 is validated for use in FC, IH for the detection of VCAM-1.
Physical form
> 95% purified immunoglobulin as determined by SDS-gel electrophoresis prior to addition of stabilizer and preservative. Liquid in PBS.
Format: Purified
Storage and Stability
Maintain at +2-8°C upon date of receipt. For long-term storage, aliquot and store at -20°C. Avoid repeated Freeze/thaw cycles.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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