MAB201P
Mouse Anti-Rabbit light chain, HRP conjugate Antibody
Chemicon®, from mouse
Synonym(s):
HRP Anti-Rabbit, Light Chain Detection Antibody, Rabbit Light Chain Detection
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
conjugate
peroxidase conjugate
antibody form
purified antibody
antibody product type
secondary antibodies
clone
monoclonal
species reactivity
rabbit
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG1κ
shipped in
wet ice
target post-translational modification
unmodified
General description
Antibody molecules typically comprise two immunoglobulin light chains covalently bound to a pair of heavy chains. Immunoglobulin light chains occur in two types, designated by the Greek letters kappa and lambda. Kappa and gamma light chains are approximately 250 amino acids in length with an average mass of about 25 kDa. The ratio of kappa to lambda found in the immunoglobulin population varies by species.
Specificity
Minimal cross-reaction with bovine, goat, Armenian hamster, horse, human, mouse, rat, and sheep.
The antibody reacts strongly with native primary antibodies primarily with kappa light chains. It is not suitable for detecting lambda light chains. The antibody does not react with the heavy chain of rabbit IgG. The antibody has been tested by ELISA and adsorbed to ensure minimal cross-reaction with bovine, goat, Armenian hamster, horse, human, mouse, rat, and sheep immunoglobulins.
Immunogen
Epitope: Kappa light chain
Prepared from rabbit IgG light chain.
Application
Detect Rabbit light chain using this Mouse anti-Rabbit light chain, HRP conjugate Antibody validated for use in ELISA, WB, IC, IH.
Research Category
Secondary & Control Antibodies
Secondary & Control Antibodies
Research Sub Category
Fragment Specific Secondary Antibodies
Fragment Specific Secondary Antibodies
Western Blotting: 1:5,000 -1:100,000 with chromogenic substrates 1:10,000 -1:200,000 with ECL substrates
ELISA: 1:5,000 -1:100,000
Immunohistochemistry: 1:500 - 1:5,000
Immunocytochemistry: 1:500 - 1:5,000
Optimal working dilutions must be determined by the end user.
ELISA: 1:5,000 -1:100,000
Immunohistochemistry: 1:500 - 1:5,000
Immunocytochemistry: 1:500 - 1:5,000
Optimal working dilutions must be determined by the end user.
Target description
25 kDa
Physical form
Purified from antiserum by chromatographic procedures. Lyophilized in 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6. 15 mg/mL BSA as stabilizer.
Serum
Storage and Stability
Maintain lyophilized product at 2°-8°C for up to 12 months. After reconstitution the product is stable for six weeks at 2°-8°C in the dark as an undiluted liquid. Reconstitute vial with of distilled water (addition of 0.5 ml water will yield a final concentration of 1 mg/mL). For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze thaw cycles. Keep away from light.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Asunción Muñoz-Silvestre et al.
The American journal of pathology, 190(6), 1188-1210 (2020-03-24)
Despite the enormous efforts made to achieve effective tools that fight against Staphylococcus aureus, the results have not been successful. This failure may be due to the absence of truly representative experimental models. To overcome this deficiency, the present work
María Rodríguez-Muñoz et al.
Translational psychiatry, 7(12), 1291-1291 (2017-12-19)
The activity of certain G protein-coupled receptors (GPCRs) and of glutamate N-Methyl-D-aspartate receptors (NMDARs) is altered in both schizophrenia and depression. Using postmortem prefrontal cortex samples from subjects with schizophrenia or depression, we observed a series of opposite changes in
Aneta Dobierzewska et al.
PloS one, 11(6), e0157221-e0157221 (2016-06-11)
Preeclampsia (PE), is a serious pregnancy disorder characterized in the early gestation by shallow trophoblast invasion, impaired placental neo-angiogenesis, placental hypoxia and ischemia, which leads to maternal and fetal morbidity and mortality. Here we hypothesized that angiogenic sphingosine kinase-1 (SPHK1)/sphingosine-1-phosphate
Nomi Pernicone et al.
Molecules (Basel, Switzerland), 27(3) (2022-02-16)
DNA-damaging chemotherapy agents such as cisplatin have been the first line of treatment for cancer for decades. While chemotherapy can be very effective, its long-term success is often reduced by intrinsic and acquired drug resistance, accompanied by chemotherapy-resistant secondary malignancies.
Jürgen Fritsch et al.
Oncotarget, 7(46), 75774-75789 (2016-10-08)
During apoptosis induction by TNF, the extrinsic and intrinsic apoptosis pathways converge at the lysosomal-mitochondrial interface. Earlier studies showed that the lysosomal aspartic protease Cathepsin D (CtsD) cleaves Bid to tBid, resulting in the amplification of the initial apoptotic cascade
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service