MAB16200-AF647

Anti-MGMT Antibody, clone MT 3.1, Alexa Fluor^™ 647 Conjugate

clone MT3.1, from mouse, ALEXA FLUOR^™ 647

• Synonym(s): Methylated-DNA-protein-cysteine methyltransferase, 6-O-methylguanine-DNA methyltransferase, GATase, MGMT, O-6-methylguanine-DNA-alkyltransferase
• UNSPSC Code: 12352203
• eCl@ss: 32160702

Properties

• biological source: mouse
• Quality Level: 100
• conjugate: ALEXA FLUOR^™ 647
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: MT3.1, monoclonal
• species reactivity: human
• technique(s): flow cytometry: suitable; immunocytochemistry: suitable
• isotype: IgG1
• NCBI accession no.: NM_002412.2
• UniProt accession no.: P16455
• shipped in: ambient
• target post-translational modification: unmodified
• Gene Information: human ... MGMT(4255)

Description

General description

Methylated-DNA-protein-cysteine methyltransferase (EC 2.1.1.63; UniProt P16455; also known as 6-O-methylguanine-DNA methyltransferase, GATase, MGMT, O-6-methylguanine-DNA-alkyltransferase) is encoded by the MGMT gene (Gene ID 4255) in human. MGMT is a DNA repair enzyme that removes alkyl groups from O6-guanine and plays a fundamental role in maintaining genomic integrity. MGMT converts the naturally occurring mutagenic DNA lesion O6-methylguanine back to guanine, thereby prevents mismatch and errors during DNA replication and transcription. MGMT expression level correlates with cellular resistance to alkylnitrosoureas, compounds that induce O6-alkylguanine lesions. MGMT transcription is negatively regulated via promoter methylation, MGMT promoter methylation level is reported to correclate with the survival rate of glioblastoma patients receiving treatment with alkylating agents.

Specificity

Clone MT 3.1 detected p53 expression-dependent MGMT downregulation in IMR90 cells (Harris, L.C., et al. (1996). Cancer Res. 56(9):2029-2032).

Immunogen

GATase purified from CEM-CCRF human leukemic lymphoblasts (Harris, L.C., et al. (1996). Cancer Res. 56(9):2029-2032; Brent, T.P., et al. (1990). Cancer Res. 50(1):58-61).

Application

Anti-MGMT Antibody, clone MT 3.1, Alexa Fluor^™ 647 Conjugate, Cat. No. MAB16200-AF647, is a highly specific mouse monoclonal antibody that targets MGMT and has been tested in Flow Cytometry and Immunocytochemistry.

Flow Cytometry Analysis: 0.2 µL from a representative lot detected MGMT in one million MCF-7 cells.

This monoclonal antibody is also available in unconjugated and Alexa Fluor^™ 488-conjugated forms (Cat. No. MAB16200 and MAB16200-AF488) for flow cytometry, immunocytochemistry, immunohistochemistry, immunoprecipitation, and Western blotting applications.

Research Category
Epigenetics & Nuclear Function

Quality

Evaluated by Immunocytochemistry in MCF-7 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this Alexa Fluor^™ 647-conjugated antibody detected MGMT in MCF-7 cells.

Target description

21.65 kDa calculated. ~25 kDa reported (Brent, T.P., et al. (1990). Cancer Res. 50(1):58-61).

Physical form

Protein A purified.

Purified mouse IgG1 Alexa Fluor^™ 647 conjugate in PBS with 1.5% BSA and 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable