MAB16200-AF488

Anti-MGMT Antibody, clone MT 3.1, Alexa Fluor^™ 488 Conjugate

clone MT3.1, from mouse, ALEXA FLUOR^™ 488

• Synonym(s): Methylated-DNA-protein-cysteine methyltransferase, 6-O-methylguanine-DNA methyltransferase, GATase, MGMT, O-6-methylguanine-DNA-alkyltransferase
• UNSPSC Code: 12352203
• eCl@ss: 32160702

Properties

• biological source: mouse
• Quality Level: 100
• conjugate: ALEXA FLUOR^™ 488
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: MT3.1, monoclonal
• species reactivity: human
• technique(s): flow cytometry: suitable; immunocytochemistry: suitable
• isotype: IgG1
• NCBI accession no.: NM_002412.2
• UniProt accession no.: P16455
• shipped in: ambient
• target post-translational modification: unmodified
• Gene Information: human ... MGMT(4255)

Description

General description

Methylated-DNA-protein-cysteine methyltransferase (EC 2.1.1.63; UniProt P16455; also known as 6-O-methylguanine-DNA methyltransferase, GATase, MGMT, O-6-methylguanine-DNA-alkyltransferase) is encoded by the MGMT gene (Gene ID 4255) in human. MGMT is a DNA repair enzyme that removes alkyl groups from O6-guanine and plays a fundamental role in maintaining genomic integrity. MGMT converts the naturally occurring mutagenic DNA lesion O6-methylguanine back to guanine, thereby prevents mismatch and errors during DNA replication and transcription. MGMT expression level correlates with cellular resistance to alkylnitrosoureas, compounds that induce O6-alkylguanine lesions. MGMT transcription is negatively regulated via promoter methylation, MGMT promoter methylation level is reported to correclate with the survival rate of glioblastoma patients receiving treatment with alkylating agents.

Specificity

Clone MT 3.1 detected p53 expression-dependent MGMT downregulation in IMR90 cells (Harris, L.C., et al. (1996). Cancer Res. 56(9):2029-2032).

Application

Anti-MGMT Antibody, clone MT 3.1, Alexa Fluor^™ 488 Conjugate, Cat. No. MAB16200-AF488, is a highly specific mouse monoclonal antibody that targets MGMT and has been tested in Flow Cytometry and Immunocytochemistry.

Flow Cytometry Analysis: 0.2 µL from a representative lot detected MGMT in one million MCF-7 cells.

This monoclonal antibody is also available in unconjugated and Alexa Fluor^™ 647-conjugated forms (Cat. No. MAB16200 and MAB16200-AF647) for flow cytometry, immunocytochemistry, immunohistochemistry, immunoprecipitation, and Western blotting applications.

Quality

Evaluated by Immunocytochemistry in MCF-7 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this Alexa Fluor^™ 488-conjugated antibody detected MGMT in MCF-7 cells.

Target description

21.65 kDa calculated. ~25 kDa reported (Brent, T.P., et al. (1990). Cancer Res. 50(1):58-61).

Physical form

Purified mouse IgG1 Alexa Fluor^™ 488 conjugate in PBS with 1.5% BSA and 0.05% sodium azide.

Other Notes

Concentration: Please refer to lot specific datasheet.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable