MAB1076-I

Anti-DYNLT1 Antibody, clone 7A9F4H10

clone 7A9F4H10, from mouse

• Synonym(s): Dynein light chain Tctex-type 1, Protein CW-1, T-complex testis-specific protein 1 homolog
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 7A9F4H10, monoclonal
• species reactivity: human
• technique(s): immunohistochemistry: suitable; western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_006510
• UniProt accession no.: P63172
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... DYNLT1(6993)

Description

General description

Eukaryotic cells rely on actin and microtubule-based protein "motors" to generate intracellular movements. These motor proteins contain specialized domains that hydrolyze ATP to produce force and movement along a cytoskeletal polymer (microtubules in the case of the kinesin family and dyneins). In all cell types, dynein is a large protein complex composed of six distinct subunits and is responsible for most intracellular movement toward the minus ends of microtubules. Cytoplasmic dynein has many important roles in transporting membrane bound organelles, including endosomes, lysosomes, and mitochondria. It is also involved in the positioning of the centrosome, the nucleus, and the Golgi. Cytoplasmic dynein is also responsible for virus transport to the nucleus, retrograde axonal transport, and microtubule and neurofilament transport. Three families of dynein light chains (DYNLT) have been identified. Two isoforms of the DYNLT family of light chains, DYNLT1 and DYNLT3, have been proposed to link dynein to specific cargoes. Recent publications indicate that DYNLT1 binds to Rhodopsin and Doc2 whereas DYNLT3 does not.

Immunogen

Recombinant protein corresponding to human DYNLT1.

Application

Detect Dynein using this mouse monoclonal antibody, Anti-DYNLT1 Antibody, clone 7A9F4H10 validated for use in western blotting & IHC.

Immunohistochemistry Analysis: A 1:100 dilution from a representative lot detected DYNLT1 human kidney and lung tissues.

Research Category
Cell Structure

Research Sub Category
Cytoskeleton

Quality

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected DYNLT1 in 10 µg of HEK293 cell lysate.

Target description

~12 kDa observed

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HEK293 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable