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IPVH304F0

Millipore

Immobilon® -P PVDF Membrane

10 sheets, 26 cm x 26 cm, 0.45 µm pore size, transfer membrane

Synonym(s):

Western blotting membrane, blotting membrane, transfer membrane

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About This Item

UNSPSC Code:
41105339
eCl@ss:
32031602
NACRES:
NB.22

product name

Immobilon®-P PVDF Membrane, 10 sheets, 26 cm x 26 cm, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.

material

PVDF membrane
plain filter
white filter

Quality Level

400

feature

hydrophobic

manufacturer/tradename

Immobilon®

technique(s)

dot blot: suitable
western blot: suitable

filter L × W

26 cm × 26 cm

pore size

0.45 μm pore size

capacity

160 μg/cm2 adsorption capacity (insulin)
 215 μg/cm2 adsorption capacity (BSA)
 294 μg/cm2 adsorption capacity (goat IgG)

compatibility

for use with Amido black
for use with CPTS
for use with Colloidal gold
for use with Coomassie brilliant blue
for use with India ink
for use with Ponceau-S red
for use with Sypro<TMSYMBOL></TMSYMBOL> ruby
for use with Toluidine blue
for use with Transillumination

detection method

chemiluminescent
colorimetric
fluorometric
radioactive

shipped in

ambient

General description

Immobilon®-P PVDF Membrane is an original, and most used, polyvinylidene fluoride (PVDF) transfer membrane for western blotting. It has a 0.45 μm pore size and is recommended for blotting proteins >20 kDa. The Immobilon®-P membrane has excellent protein retention, high physical strength, and broad chemical compatibility, making it ideal for a variety of staining applications and reprobing in immunodetection methods.

Application

Immobilon®-P PVDF Membrane has been used in western blotting.

Features and Benefits

  • Compatible with a variety of detection methods, including radioactive, chromogenic, and chemiluminescent techniques.
  • High protein adsorption and retention ensures greater sensitivity
  • Devoid of crack or curl like nitrocellulose. Can be cut without fracturing and reprobed multiple times.
  • Eliminates the need for blocking and reduces detection times by up to 2 hours.

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Tomohiko Mori et al.
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Tumor necrosis factor-related, apoptosis-inducing ligand (TRAIL) is a potent inducer of apoptosis in a wide variety of tumor cells, but it does not cause toxicity in the majority of normal cells. Therefore, TRAIL could become a suitable agent for anticancer
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We aimed to investigate the involvement of macroautophagy/autophagy in autoimmunity in rheumatoid arthritis (RA) through citrullination of VIM (vimentin) and its interaction with MHC class II in synovial fibroblasts (SFs). The cell surface expression of MHC class II and B7
Maria C Todd et al.
Oncology letters, 10(1), 156-162 (2015-07-15)
Tumor-specific deregulated expression of claudins, integral membrane proteins found in tight junctions (TJs), has indicated a possible role for TJ disruption in cancer progression. The current study demonstrates the marked overexpression of claudin-3 protein in two breast cancer cell lines

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