IM42

Anti-MT1-MMP (Ab-3) Mouse mAb (114-6G6)

liquid, clone 114-6G6, Calbiochem^®

• Synonym(s): Anti-MMP-14
• UNSPSC Code: 12352203

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 114-6G6, monoclonal
• form: liquid
• contains: ≤0.1% sodium azide as preservative
• species reactivity: human
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• isotype: IgG1
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... MMP14(4323)

Description

General description

Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS1 mouse myeloma cells. Recognizes the ~66 kDa latent and the ~60 kDa active forms of MT1-MMP protein.

Recognizes the ~60-66 kDa MTI-MMP protein in HT-1080 cells and ConA-treated MD-MB-231 cells.

This Anti-MT1-MMP (Ab-3) Mouse mAb (114-6G6) is validated for use in Frozen Sections, Paraffin Sections, Immunoblotting, Immunocytochemistry, IP for the detection of MT1-MMP (Ab-3).

Immunogen

a synthetic peptide (REVPYAYIREGHEK) corresponding to amino acids 160-173 in the catalytic domain of human MT1-MMP

Application

Frozen Sections (not recommended)
Paraffin Sections (10 g/ml, Pronase<TMSYMBOL></TMSYMBOL> or heat pre-treatment required)
Immunoblotting (10 g/ml)
Immunocytochemistry (see application reference)
Immunoprecipitation (not recommended)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Negative Control
trpE (Ab-1) or untreated MD-MB-231 cells

Positive Control
HT-1080 cells, ConA-treated MD-MB-231 cells, or breast carcinoma tissue

Other Notes

Does not cross-react with MT2-MMP or MT3-MMP. To induce MT1-MMP in MD-MB-231 cells, treat with 1-5 µg/ml ConA for 24-48 h. For best results with paraffin sections, fix with perioddate-lysine-4% paraformaldehyde. Antibody should be titrated for optimal results in individual systems.

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Okada, A., et al. 1997. J. Cell. Biol.147, 67.
Okumura, Y., et al. 1997. FEBS Lett.402, 181.
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Takino, T., et al. 1995. J. Biol. Chem.270, 23013.
Sato, H., et al. 1994. Nature370, 61.
Cottam, D.W. and Rees, R.C. 1993. Intl J. Oncol.2, 861.
Stetler-Stevenson, W.G., et al. 1993. FASEB J.7, 1434.
Fridman, R., et al. 1992. J. Biol. Chem.267, 15389.
Woessner, J.F. 1991. FASEB J.5, 2145.
Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Sem. Cancer Biol., ed. M.M. Gottesman. Vol. 1(2):99-106.
Goldberg, G.I., et al. 1989. PNAS86, 8207.

Legal Information

Manufactured by Daiichi Fine Chemical Co., Ltd. Not available for sale in Japan.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1