IHCR2014-6

IHC Select Anti-Neuron-Specific Enolase Antibody, prediluted, clone BBS/NC/VI-H14

clone BBS/NC/VI-H14, from mouse

• Synonym(s): NSE
• UNSPSC Code: 12352203
• eCl@ss: 32160702

Properties

• biological source: mouse
• antibody form: tissue culture supernatant
• antibody product type: primary antibodies
• clone: BBS/NC/VI-H14, monoclonal
• species reactivity: human
• manufacturer/tradename: Chemicon^®; IHC Select
• technique(s): immunohistochemistry: suitable (paraffin)
• isotype: IgG1κ
• NCBI accession no.: NM_001975.2
• UniProt accession no.: P09104
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... ENO2(2026)

Description

Specificity

Reacts with human gamma-gamma enolase (Mr 46000). This antibody does not cross react with alpha or beta subunits of NSE.

Immunogen

Gamma-gamma enolase purified from human brain

Application

Antibody is prediluted and ready to use for Immunohistochemistry of formalin-fixed, paraffin-embedded tissues.

Pretreatment: Heat Induced Epitope Retrieval (HIER). Recommend Citrate Buffer, pH 6.0 (Cat. No. 21545). No signal was detected without Epitope retrieval.

Incubation: 10 minutes with IHC Select Detection Kits.

NSE has been prediluted for use as the primary antibody with Chemicon′s IHC Select Detection Kits and Protocols (Catalog Nos. DAB050, DET-HP1000, APR050, and DET-APR1000), but other supplier′s IHC detection systems may be used. For optimized protocol details, visit www.chemicon.com and select the protocols link under Cat. No.IHCR2014-6.

This IHC Select Anti-Neuron-Specific Enolase Antibody, prediluted, clone BBS/NC/VI-H14 is validated for use in IH(P) for the detection of Neuron-Specific Enolase.

Physical form

Format: Purified

Monoclonal mouse antibody supplied in liquid format as tissue culture supernatant, diluted in PBS, pH 7.2 with stabilizers, 0.2% Tween 20, and 0.1% Kathon as a preservative.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H317,H412
• pcodes: P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313
• Hazard Classifications: Aquatic Chronic 3 - Skin Sens. 1
• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable