GF109
Ciliary Neurotrophic Factor Protein, Recombinant human
The human Ciliary Neurotrophic Factor (or CNTF protein) is a potent neural factor that was originally characterized as a survivability factor for chick ciliary neurons in vitro.
Synonym(s):
CNTF
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About This Item
UNSPSC Code:
12352200
eCl@ss:
32160405
NACRES:
NA.75
Recommended Products
biological source
human
Quality Level
assay
>98% (SDS-PAGE and HPLC)
manufacturer/tradename
Chemicon®
impurities
<0.1 ng/μg Endotoxin (of CNTF; 1EU/ug)
NCBI accession no.
UniProt accession no.
shipped in
dry ice
Gene Information
human ... CNTF(1270)
General description
Ciliary Neurotrophic Factor (CNTF) is a potent neural factor that was originally characterized as a survivability factor for chick ciliary neurons in vitro. CNTF also has been shown to promote survivability and differentiation of other neural cell types. Recombinant Human CNTF is a 22.7 kDa protein containing 199 amino acid residues.
Product Source: E.coli
Biochem/physiol Actions
The ED50 as determined by the dose-dependent proliferation of human TF-1 cells is 50-150 ng/mL.
Physical form
The sterile filtered solution was lyophilized from 10 mM Citric Acid, pH 3.0 + 75 mM sodium chloride.
Storage and Stability
The lyophilized powder is stable for a few weeks at room temperature, but best stored at -20°C. Reconstitute in 5 mM NaP, pH 7.5 to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use. Reconstituted human CNTF should be stored in undiluted aliquots at -20°C. Avoid repeated freeze/thaw cycles.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
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S Emami et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 15(2), 351-361 (2001-02-07)
Trefoil factors (TFFs) are protease-resistant peptides that promote epithelial cell migration and mucosal restitution during inflammatory conditions and wound healing in the gastrointestinal tract. To date, the molecular mechanism of TFFs action and their possible role in tumor progression are
PKCepsilon induces astrocyte stellation by modulating multiple cytoskeletal proteins and interacting with Rho A signalling pathways: implications for neuroinflammation.
Miguel Burgos,Soledad Calvo,Francisca Molina,Cecilia F Vaquero,Allen Samarel,Juan Llopis et al.
The European Journal of Neuroscience null
CNTF reverses obesity-induced insulin resistance by activating skeletal muscle AMPK
Watt, Matthew J, et al
Nature Medicine, 12, 541-548 (2006)
S Rodrigues et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 15(9), 1517-1528 (2001-06-28)
We have investigated the possible functional relationships between cellular invasion pathways induced by trefoil factors (TFFs), src, and the cyclooxygenases COX-1 and COX-2. Pharmacological inhibitors of the Rho small GTPase (C3 exoenzyme), phospholipase C (U-73122), cyclooxygenases (SC-560, NS-398), and the
J S Fine et al.
Inflammation, 25(2), 61-67 (2001-04-26)
The intracellular signaling pathways involved in human monocyte chemotaxis toward a variety of chemoattractant molecules were evaluated using selected pharmacological agents. Neither phosphatidylinositol-3-kinase (P13K) or extracellular signal-regulated kinase (ERK) activity were required for monocyte migration toward monocyte chemoattractant protein-1 (MCP-1)
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