FCMAB227F

Milli-Mark^® Anti-mouse CD3-FITC Antibody, clone 145-2C11

clone 145-2C11, Milli-Mark^®, from hamster

• Synonym(s): T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: hamster
• conjugate: FITC conjugate
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 145-2C11, monoclonal
• species reactivity: mouse
• manufacturer/tradename: Milli-Mark^®
• technique(s): flow cytometry: suitable
• isotype: IgG
• NCBI accession no.: NP_031674.1
• UniProt accession no.: P22646
• shipped in: wet ice
• Gene Information: mouse ... Cd3E(12501)

Description

General description

Human TCR/CD3 is a complex structure on the lymphocyte surface. It consists of the TCR alpha beta or TCR gamma delta heterodimer and the associated CD3 complex. The CD3 complex is composed of six polypeptides with usually four different transmembrane CD3 chains, gamma, delta, epsilon. and zeta. Three different dimers, gamma epsilon, delta epsilon, and zeta zeta, constitute the CD3 complex. The MW of CD3epsilon is 20 000 (Campana, 1987). CD3 is first detectable in early thymocytes and its appearance probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, during early stages of maturation, the CD3 antigen is predominantly present in the cell cytoplasm. In medullary thymocyles, the CD3 antigen is predominantly detected on the cell surface (Swerdlow, 1988; Beverley, 1981). The majority of T cell neoplasms also expresses CD3, but it is absent from non-T-cell lymphoid malignancies (McMichael, 1987). Consistent with the pattern of synthesis of the antigen in normal thymocytes, the earliest site detectable within neoplastic cells is the cell cytoplasm (Swerdlow, 1988).

Specificity

Antibody recognizes CD3 epsilon chain.

Immunogen

Animals were immunized with the BM10-37 mouse cytotoxic T lymphocyte (CTL) cell clone (anti H-2 Kb). Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

Application

Research Category
Inflammation & Immunology

Research Sub Category
Immunoglobulins & Immunology

This Milli-Mark Anti-mouse CD3-FITC Antibody, clone 145-2C11 is validated for use in FC for the detection of mouse CD3.

Quality

Evaluated by flow cytometry using mouse splenocytes

Target description

21 kDa Calculated

Physical form

Protein G Purified

Purified hamster monoclonal IgG conjugated to FITC in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Storage and Stability

Maintain refrigerated at 2-8°C protected from light for up to 6 months from date of receipt.

Analysis Note

Control
Mouse splenocytes

Legal Information

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10-13 - German Storage Class 10 to 13