FCMAB129CP

Milli-Mark Anti-EGFR-PerCP Antibody, clone LA22

clone LA22, Milli-Mark^®, from mouse

• Synonym(s): Receptor tyrosine-protein kinase ErbB-1 ; ; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog ; ; cell growth inhibiting protein 40 ; ; cell proliferation-inducing protein 61 ; ; epidermal growth factor receptor
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: Peridinin-Chlorophyll-Protein Complex
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: LA22, monoclonal
• species reactivity: human
• manufacturer/tradename: Milli-Mark^®
• technique(s): flow cytometry: suitable
• isotype: IgG2a
• NCBI accession no.: NP_005219.2
• UniProt accession no.: P00533
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... EGFR(1956)

Description

General description

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. The protein kinase family is one of the largest families of proteins in eukaryotes, classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. Epidermal Growth factor receptor (EGFR) is the prototype member of the type 1 receptor tyrosine kinases. EGFR overexpression in tumors indicates poor prognosis and is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.

Specificity

Recognizes the sequence of Ala351-Asp364 of human EGF receptor, Mr 180kDa, (based on numbering without the signal peptide) (Wu et al., 1989).

Immunogen

A431 membrane proteins

Epitope: Unknown

Application

Working Solution	Cells / test	Total Reaction Volume
5 μl	5 x 105 Cells / 95 μl PBS	100 μl
10 μl	1 x 106 Cells / 90 μl PBS	100 μl

Milli-Mark Anti-EGFR-PerCP Antibody, clone LA22 detects level of EGFR & has been published & validated for use in FC.

Research Category
Signaling

Research Sub Category
Growth Factors & Receptors

Quality

Evaluated by flow cytometry using A431 cells.

Target description

180 kDa Observed

Physical form

Protein G Purified

Purified mouse monoclonal IgG2a conjugated to PerCP in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Storage and Stability

Maintain refrigerated at 2-8 oC protected from light in undiluted aliquots for up to 6 months from date of receipt.

Analysis Note

Control
A431 cells.

Legal Information

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable