FCMAB115F
Anti-TRA-1-60 Antibody, clone TRA-1-60, FITC conjugate
clone TRA-1-60, from mouse, FITC conjugate
About This Item
Recommended Products
biological source
mouse
Quality Level
conjugate
FITC conjugate
antibody form
purified antibody
antibody product type
primary antibodies
clone
TRA-1-60, monoclonal
species reactivity (predicted by homology)
human (based on 100% sequence homology)
technique(s)
flow cytometry: suitable
immunocytochemistry: suitable
isotype
IgM
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... PODXL(5420)
General description
Specificity
No immunoreactivity is seen with murine EC, EG, or ES cells. Both the TRA-1-60 and TRA-1-81 monoclonal antibodies (MAB4381) recognize antigens that are associated with a pericellular matrix proteoglycan. TRA-1-60 reacts with a sialidase-sensitive epitope whilst TRA-1-81 reacts with an unknown epitope of the same molecule
Immunogen
Application
Immunocytochemical staining of live H9 human ES cells incubated for 30 minutes at 37C with 1:100 dilution of anti-TRA-1-60 FITC conjugated (Cat. No. FCMAB115F) monoclonal antibody. Pluripotent human ES cells exhibit strong immunoreactivity to this antibody.
Flow Cytometry:
Antibody dilution for cellular staining:
- Prepare an antibody working solution by diluting 1:5 the primary antibody with PBS.
- Dispense the volume per test of working of solution according to the number of cells indicated in the table below.
- 5 L for Guava Flow Cytometer
- 10 L for other Flow Cytometry instruments
5 μl Working Solution: 5 x 105 Cells / 95 μl PBS. 100 μl Total Reaction Volume
10 μl Working Solution: 1 x 106 Cells / 90 μl PBS. 100 μl Total Reaction Volume
Stem Cell Research
Pluripotent & Early Differentiation
Target description
Physical form
Storage and Stability
Analysis Note
Pluripotent human embryonic stem (ES) cells
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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