FCMAB108A4

Anti-phospho-SMC1 (Ser957) Antibody, clone 5D11G5 Alexa Fluor^™ 488

clone 5D11G5, from mouse, ALEXA FLUOR^™ 488

• Synonym(s): CDLS2, DKFZp686L19178, DXS423E, KIAA0178, MGC138332, OTTHUMP00000061876, SB1.8, SMC1, SMC1A, SMC1L1, SMC1alpha, SMCB, Sb1.8, Smcb
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• conjugate: ALEXA FLUOR^™ 488
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 5D11G5, monoclonal
• species reactivity: human, Xenopus, bovine
• technique(s): flow cytometry: suitable; western blot: suitable
• isotype: IgG1
• NCBI accession no.: NM_006306
• UniProt accession no.: Q14683
• shipped in: wet ice
• target post-translational modification: phosphorylation (pSer957)
• Gene Information: human ... SMC1A(8243)

Description

General description

SMC (structural maintenance of chromosomes) proteins are Involved in chromosome cohesion during cell cycle and in DNA repair. ATM phosphorylates SMC1 on Ser957 and Ser966 both in vitro and in vivo. Phosphorylated SMC1 is part of the complex that functions as a downstream effector in both the ATM/NBS1 branch and the ATR/MSH2 branch of S-phase checkpoint.

Specificity

Antibody recognizes SMC1 phosphorylolated at Ser957.

Predicted to cross-react with xenopus and bovine based on sequence homology

Immunogen

Epitope: Phosphorylated at and around Ser957

KLH-conjugated, synthetic peptide corresponding to human SMC1 phosphorylated at Ser957

Application

Research Category
Epigenetics & Nuclear Function

Apoptosis & Cancer

Research Sub Category
Cell Cycle, DNA Replication & Repair

This Anti-phospho-SMC1 (Ser957) Antibody, clone 5D11G5 Alexa Fluor 488 is validated for use in WB, FC for the detection of phospho-SMC1 (Ser957).

Quality

Evaluated by Flow Cytometry with Hela Cells

Target description

150kDa Calculated

Physical form

Protein G Purified

Purified mouse monoclonal IgG1 conjugated to Alexa Flour 488 in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Storage and Stability

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 6 months from date of receipt. Protect from light.

Analysis Note

Control
HeLa Cells

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable