Skip to Content
MilliporeSigma
All Photos(1)

Key Documents

ES-006

Millipore

EmbryoMax® Gelatin Solution

0.1% Gelatin Solution is available in a 500 mL format and may be used for routine mouse embryonic stem cell culture applications.

Synonym(s):

Porcine Gelatin Coating Solution, porcine gelatin

Sign Into View Organizational & Contract Pricing

Select a Size

500 ML
$41.10

$41.10

In Stock

Request a Bulk Order
All Photos(1)

Select a Size

Change View
500 ML
$41.10

About This Item

UNSPSC Code:
12352207
eCl@ss:
32160801
NACRES:
NA.75

$41.10

In Stock

Request a Bulk Order

Product Name

EmbryoMax® 0.1% Gelatin Solution, The EmbryoMax 0.1% Gelatin Solution is available in a 500 mL format and may be used for routine mouse embryonic stem cell culture applications.

Quality Level

100
200

form

liquid

manufacturer/tradename

Specialty Media
EmbryoMax®

technique(s)

cell culture | stem cell: suitable

input

sample type induced pluripotent stem cell(s)
sample type: mouse embryonic stem cell(s)

Application

Procedure for Coating Multiwell Dishes

1. Add the appropriate amounts of 0.1% Gelatin in Sterile Water to each well of the plate as indicated in the table below:

Wells_Amount/Well
96100 μL
48300 μL
240.5 mL
121.0 mL
61.5-2.0 mL

2. Leave the gelatin solution on the wells for at least 30 minutes at room temperature, with dish lids on.

3. Remove the gelatin solution and discard.

4. Immediately add cells to the dish.

Note: Do not allow the dishes to dry before adding cells.

Physical form

EmbryoMax 0.1% Gelatin Solution. Gelatin is sourced from porcine. Product is ready to use for plating.

Storage and Stability

Store at ambient temperature for upto 2 years

Legal Information

EmbryoMax is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Articles

Embryo Culture Media

Mouse embryo media and embryo validated reagents for transgenic mouse embryo culture

Protocols

Mouse Embryonic Stem Cell Culture Procedures & Protocols

Stem Cell protocols for cryopreservation, thawing of cryopreserved stem cells and media preparation.

Induced Pluripotent Stem Cell Reprogramming Protocols

Stem cell reprogramming protocols to generate human induced pluripotent stem cells (iPSCs) including viral and non-viral RNA based methods.

Questions

  1. if we are using treated flasks (like crystal-clear polystyrene flasks or Poly-D- lysine coated flasks) then still we have to use gelatin or not?

    1 answer

    1. Whether the need to use gelatin in cell culture will depend on the specific requirements of the cells and the experimental goals. Gelatin is often used as a coating material to enhance cell adhesion in some cell culture applications even with cell culture treated flasks.

      To further discuss, we kindly ask you to navigate to the link https://www.sigmaaldrich.com/techservice, click on "Product Technical Inquires" under the Products Section with all the required information so that a member of our team can reach out to you to assist further. Thank you.

      Helpful?

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service