CP11L
Anti-Integrin α₃ Mouse mAb (P1B5)
lyophilized, clone P1B5, Calbiochem®
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About This Item
UNSPSC Code:
12352203
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
P1B5, monoclonal
form
lyophilized
does not contain
preservative
species reactivity
human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
isotype
IgG1
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... ITGA3(3675)
General description
Purified mouse monoclonal antibody generated by immunizing RBF/Dn mice with the specified immunogen and fusing splenocytes with NS-1/FOX-NY mouse myeloma cells (see application references). Recognizes the α3 integrin subunit.
Recognizes the α3-subunit of the α3/β1 integrin complex protein in human mononuclear cells.
This Anti-Integrin α₃ Mouse mAb (P1B5) is validated for use in Cell Inhibition Assays, FC, IF, IP for the detection of Integrin α₃.
Immunogen
Human
human mononuclear cells
Application
Cell Inhibition Assays (1-5 µg/ml, see application references)
Flow Cytometry (1-5 µg/ml)
Immunoblotting (not recommended)
Immunofluorescence (1-5 µg/ml)
Immunoprecipitation (1-2 µg/sample)
Paraffin Sections (not recommended)
Flow Cytometry (1-5 µg/ml)
Immunoblotting (not recommended)
Immunofluorescence (1-5 µg/ml)
Immunoprecipitation (1-2 µg/sample)
Paraffin Sections (not recommended)
Warning
Toxicity: Standard Handling (A)
Physical form
Lyophilized from a volatile buffer, 100 µg BSA.
Reconstitution
We recommend resuspending the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage (with azide) or aliquot and freeze (-20°C) for short-term storage.
Analysis Note
Positive Control
A2058 cells
A2058 cells
Other Notes
Albelda, S.M. and Buck, C.A. 1990. FASEB J.4, 2868.
Cheresh, D.A. et al. 1990. Cell57, 59.
Krissansen, G.W. et al. 1990. J. Biol. Chem.265, 823.
Vogel, B, et al. 1990. J. Biol. Chem.265, 5934.
Wayner, E.A. et al.1988 J. Cell Biol.107, 1881.
Buck, C.A. and Horwitz, A.F. 1987. Rev. Cell Biol.3, 179.
Hynes, R.O. 1987 Cell48, 549.
Wayner, E.A. and Carter, W.G., 1987. J. Cell Biol.105, 1873.
Cheresh, D.A. et al. 1990. Cell57, 59.
Krissansen, G.W. et al. 1990. J. Biol. Chem.265, 823.
Vogel, B, et al. 1990. J. Biol. Chem.265, 5934.
Wayner, E.A. et al.1988 J. Cell Biol.107, 1881.
Buck, C.A. and Horwitz, A.F. 1987. Rev. Cell Biol.3, 179.
Hynes, R.O. 1987 Cell48, 549.
Wayner, E.A. and Carter, W.G., 1987. J. Cell Biol.105, 1873.
Will partially inhibit cell-cell adhesion of E1L-8 cells. Antibody should be titrated for optimal results in individual systems.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Jia Jia et al.
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