CC054-M

Human Collagen Type III

• Synonym(s): alpha 1 Type III Collagen
• UNSPSC Code: 12352203
• eCl@ss: 32160405

Properties

• biological source: human
• Quality Level: 300
• assay: 90% (SDS-PAGE)
• form: liquid
• manufacturer/tradename: Chemicon^®
• technique(s): cell culture | mammalian: suitable
• impurities: <1% human collagen types II, IV-VI, and non-collagen proteins; <10% human collagen type I
• input: sample type: human embryonic stem cell(s); sample type pancreatic stem cell(s); sample type mesenchymal stem cell(s); sample type neural stem cell(s); sample type hematopoietic stem cell(s); sample type induced pluripotent stem cell(s); sample type epithelial cells
• NCBI accession no.: NM_000090.3
• UniProt accession no.: P02461

Description

General description

COL3A1 is the gene responsible for producing the pro-alpha1(III) chain of type III procollagen. Type III collagen, which adds structure and strength to connective tissues, is found in many places in the body, especially skin, lung, intestinal walls, and the walls of blood vessels. Collagen III is initially produced as procollagen, a protein consisting of three pro-alpha1(III) chains that form the triple-stranded, rope-like molecule. After being synthesized, the procollagen molecule is modified by the cell. Enzymes modify the amino acids lysine and proline in the protein strands by adding chemical groups that are necessary for the strands to form a stable molecule and then later to crosslink to other molecules outside the cell. Other enzymes add sugars to the protein. The type III procollagen molecules are released from the cell and are processed by enzymes that clip small segments off either end of the molecules to form mature collagen. The mature collagen molecules assemble into fibrils. Cross-linking between molecules produces a very stable fibril, contributing to collagen′s tissue strengthening function.{http://ghr.nlm.nih.gov}

Human placenta, negative for HbsAG, HCV and HIV 1 & 2 antibodies.

Human type III collagen purified by serial salt precipitations, alcohol precipitation and DEAE chromatography of an acetic acid extraction of human placenta, following mild pepsin digestion. Composition: [a1(III)]3, native triple helix. Ability to form native helical structure verified by ORD measurement, competence in microfibril formation and reactivity with anti-collagen type-specific monoclonal antibodies

Physical form

Liquid in 0.5 M acetic acid, pH 2.5.

Storage and Stability

Maintain at -20ºC in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.

Analysis Note

Retention of native structure was confirmed by ability to form microfibrils.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable