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CBL191

Sigma-Aldrich

Anti-Complement C3a Antibody, clone H13

clone H13, Chemicon®, from mouse

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About This Item

UNSPSC Code:
12352202
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

H13, monoclonal

species reactivity

guinea pig, rabbit, human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG2b

NCBI accession no.

NM_000064.2

UniProt accession no.

P01024

shipped in

wet ice

Gene Information

human ... C3(718)

Specificity

The antibody recognizes an epitope on the alpha-chain of Complement C3 (Western blot). The antibody inhibits the anaphylactic function of C3a: the capacity of C3a to induce serotonin release from thrombocytes in vitro is inhibited; similarly, treatment of C3a with the antibody inhibits the C3a-induced skin reaction in vivo. The antibody is, in combination with antibodies to the C3b fragment, an excellent control for distinguishing ubiquitous or nonspecifically bound native C3 from the biologically active C3 fragment. Nonactivated C3 still bears the C3a determinant, whereas activated C3 (C3b, C3d) is C3a-negative. It is therefore useful for immunopathological assays (demonstration of C3 deposition in biopsies) and for C3a assays.

Immunogen

Human complement component C3

Application

Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
This Anti-Complement C3a Antibody, clone H13 is validated for use in ELISA, IH, WB for the detection of Complement C3a.

Physical form

Format: Purified
Protein A purified immunoglobulin lyophilized from PBS, pH 7.4, containing 0.5% BSA and 0.09% sodium azide. Reconstitute with 1 mL distilled water.

Storage and Stability

Maintain at 2°-8°C for up to 12 months from date of receipt.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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GHS07

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Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 3

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Quantitation of the anaphylatoxin C3a in the presence of C3 by a novel sandwich ELISA using monoclonal antibody to a C3a neoepitope.
Zilow, G, et al.
Journal of Immunological Methods, 121, 261-268 (1989)
Momar Ndao et al.
Journal of clinical microbiology, 48(4), 1139-1149 (2010-01-15)
More than 10 million people are thought to be infected with Trypanosoma cruzi, primarily in the Americas. The clinical manifestations of Chagas' disease (CD) are variable, but most subjects remain asymptomatic for decades. Only 15 to 30% eventually develop terminal

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