CBL158
Anti-Integrin β2 [CD18] Antibody
clone MEM 48, Chemicon®, from mouse
Synonym(s):
CD18
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
MEM 48, monoclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
flow cytometry: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
Gene Information
human ... ITGB2(3689)
Specificity
This antibody recognizes the CD18 molecule, a 95 kDa integrin molecule (beta 2 integrin) that forms the common beta chain of the CD11/CD18 heterodimer integrins which are expressed on all leucocytes. The beta2 integrin is the ICAM 1 receptor
FUSION PARTNER:
Sp 2/0 - Ag 14 myeloma cell line
FUSION PARTNER:
Sp 2/0 - Ag 14 myeloma cell line
Immunogen
Leucocytes from an LGL - type leukaemia
Application
Anti-Integrin β2 [CD18] Antibody detects level of Integrin β2 [CD18] & has been published & validated for use in FC, WB, IH.
Immunohistochemistry: Identification of CD18+ leucocytes on cryostat sections
Function Blocking: Studies of cell-cell and cell-matrix adhesion, this antibody will block cell-cell interaction, (Grönholm, 2018)
Flow Cytometry
Western Blot
Optimal working dilutions must be determined by the end user.
Function Blocking: Studies of cell-cell and cell-matrix adhesion, this antibody will block cell-cell interaction, (Grönholm, 2018)
Flow Cytometry
Western Blot
Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Integrins
Integrins
Linkage
Replaces: CBL514
Physical form
Format: Purified
The monoclonal is presented in phosphate buffered saline containing 15mM sodium azide, pH 7.4. We recommend that each laboratory determine an optimum working titre for use in its particular application.
Storage and Stability
Maintain at 2o-8oC for up to 12 months from date of receipt. For long term storage aliquot antibody into small volumes and store at -20°C for up to one year.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Structure and function of leukocyte integrins.
Larson, R S and Springer, T A
Immunological Reviews, 114, 181-217 (1990)
Association of the CD59 and CD55 cell surface glycoproteins with other membrane molecules
Stefanova, I and Horejsi, V
Journal of immunology (Baltimore, Md. : 1950), 147, 1587-1592 (1991)
Monoclonal antibodies against human leucocyte antigens. IV. Antibodies against subunits of the LFA-1 (CD11a/CD18) leucocyte-adhesion glycoprotein.
Bazil, V, et al.
Folia Biologica (Praha.), 36, 41-50 (1990)
Amir E Zeituni et al.
Journal of immunology (Baltimore, Md. : 1950), 183(9), 5694-5704 (2009-10-16)
The oral mucosal pathogen Porphyromonas gingivalis expresses at least two adhesins: the 67-kDa mfa-1 (minor) fimbriae and the 41-kDa fimA (major) fimbriae. In periodontal disease, P. gingivalis associates in situ with dermal dendritic cells (DCs), many of which express DC-SIGN
K Drbal et al.
Biochemical and biophysical research communications, 275(2), 295-299 (2000-08-31)
Monoclonal antibodies to CD18, the common chain of leukocyte integrins, recognize in various types of human lymphoid and myeloid cells under the conditions of nonreducing Western blotting three species of CD18 of mol. wt. 96, 87, and 78 kDa, respectively.
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