ABT240

Anti-Ankyrin-B

from rabbit

• Synonym(s): ANK-2, ANK-2, Ankyrin-B, Brain Ankyrin, Non-erythroid Ankyrin
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: human
• technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• NCBI accession no.: NM_001148.4
• UniProt accession no.: Q01484
• shipped in: ambient
• target post-translational modification: unmodified
• Gene Information: human ... ANK2(287)

Description

General description

Ankyrin-2 (UniProt: Q01484; also known as ANK-2, Ankyrin-B, Brain Ankyrin, Non-erythroid Ankyrin) is encoded by the ANK2 gene (Gene ID: 287) in human. Most ankyrins are typically composed of three structural domains: an amino-terminal domain containing multiple ankyrin repeats; a central region with a highly conserved spectrin binding domain; and a carboxy-terminal regulatory domain, which is the least conserved and subject to variation. ANK-2 is a cytoskeletal protein that is present in plasma membrane of neurons as well as glial cells throughout the brain and it is expressed in fetal brain and in temporal cortex of adult brain. Its expression is also shown in the inner segments of rod photoreceptors in retina and at the apical membrane of airway lung epithelial cells. Four isoforms of ANK-2 have been reported that are produced by alternative splicing. ANK-2 can be phosphorylated at multiple sites by different protein kinases and each phosphorylation event regulates its structure and function. In skeletal muscle, ANK-2 is required for proper localization of DMD and DCTN4 and for the formation and/or stability of a special subset of microtubules associated with costameres and neuromuscular junctions. Mutations in ANK2 gene are known to cause Long QT syndrome 4 (LQT4), which is characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. It can cause syncope (temporary loss of consciousness caused by a fall in blood pressure) and sudden death in response to exercise or emotional stress.

Specificity

This rabbit polyclonal antibody detects Ankyrin-B in human cells. It targets an epitope within 228 amino acids from the internal region.

Immunogen

Epitope: cytoplasmic domain

GST-tagged Recombinant Protein corresponding to the cytoplasmic domain of Human Ankyrin-B.

Application

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Ankyrin-B in mouse striatum and mouse hippocampus tissues.

Western Blotting Analysis: 1 µg/mL from a representative lot detected Ankyrin-B in SK-N-SH cell lysate.

Research Category
Cell Structure

This rabbit polyclonal Anti-Ankyrin-B, Cat. No. ABT240, is a rabbit polyclonal antibody that detects Ankyrin-B and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting,

Quality

Evaluated by Immunohistochemistry in human cerebral cortex tissue.

Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected Ankyrin-B in human cerebral cortex tissue.

Target description

433.72 kDa calculated.

Physical form

Affinity Purified

Format: Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine, 0.15 M NaCl, pH7.4 with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable