Platelet endothelial cell adhesion molecule (UniProt P16284; also known as CD31, EndoCAM, GPIIA′, PECA1, PECAM-1) is encoded by the PECAM1 gene (Gene ID 5175) in human. PECAM-1 (CD31) is a 130-kD cell adhesion molecule that is expressed at the junctions of all continuous endothelia, as well as most bone marrow-derived hematopoietic cells, including platelets, monocytes, granulocytes and some lymphocytes. The human PECAM1 gene produces six spliced isoforms with varying lengths in cytoplasmic domain, with the isoform Long being the major form expressed in human tissues and endothelial cells (ECs), and isoform delta 14-15 being the predominantly expressed murine form. PECAM-1 plays an import role in maintaining vascular integrity and function by mediating ECs interactions with pericytes and smooth muscle cells (SMCs). PECAM-1 is initially produced with a signal peptide (a.a. 1-27) sequence, the removal of which yields the mature protein with a large extracellular region (a.a. 28-601) that contains six Ig-like C2-type domains, followed by a transmembrane seqment (a.a. 602-620) and a cytoplasmic tail (a.a. 621-738 of isoform Long) that plays an essential role in PECAM-1-mediated cellular signaling.
Specificity
Expected to react with all six spliced isoforms of human PECAM-1 reported by UniProt (P16284).
Immunogen
KLH-conjugated linear peptide corresponding to a cytoplasmic domain seqence of human CD31/PECAM-1.
Application
Anti-CD31/PECAM-1 Antibody is an antibody against CD31/PECAM-1. The CD31 antibody is used in Western Blotting and Flow Cytometry applications.
Flow Cytometry Analysis: 0.1 µg of this antibody from a representative lot detected CD31/PECAM-1 in HUVECs and Jurkat cells.
Quality
Evaluated by Western Blotting in HUVECs lysate.
Western Blotting Analysis: 0.25 µg/mL of this antibody detected CD31/PECAM-1 in 10 µg of HUVEC lysate.
Target description
~130 kDa observed. Target band size is larger than the calculated molecular weights due to glycosylation. 79.58 kDa (isoform Long), 77.52 kDa (isoform Delta12), 77.25 kDa (isoform Delta13), 77.46 kDa (isoform Delta14), 77.30 kDa (isoform Delta14-15), 78.42 kDa (isoform Delta15) calculated.
Other Notes
Concentration: Please refer to lot specific datasheet.
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