Ceramide synthase 5 (CerS5), also known as LAG1 longevity assurance homolog 5, and encoded by the gene name CERS5 or LASS5, belong to the LASS family representing a subgroup of the homeobox gene family and are highly conserved from yeasts to mammals. Six members of this family of proteins have been characterized (LASS1-6) and all are involved in ceramide synthesis during cell growth regulation and cancer differentiation. LASS5, also called Trh4, is a 392 amino acid endoplasmic reticulum, multi-pass membrane protein. Functioning as a dihydro-ceramide synthase, LASS5 is involved in the production of sphingolipids containing mainly one fatty acid donor (N-linked palmitoylceramide) in a fumonisin B1-independent manner. It uses palmitoyl-CoA as an acyl donor and is involved in the synthesis of C14, C16 and C18-ceramide. LASS5 is the most abundantly expressed and predominant ceramide synthase isoform in lung epithelia. Recent studies show that LASS5 partially correct growth and apoptosis. Multiple isoforms of LASS5 are known to exist. This antibody may cross-react with the highly homologous LASS6.
Immunogen
Epitope: N-terminus
KLH-conjugated synthetic peptide corresponding to the N-terminus of human LASS5.
Application
Anti-LASS5 Antibody is an antibody against LASS5 for use in western blotting, IHC & immunofluorescence.
Immunohistochemistry Analysis: 2.5 μg/mL from a representative lot detected LASS5 in human brain tissue.
Immunofluorescence Analysis: 20 μg/mL from a representative lot detected LASS5 in human brain tissue.
Research Category Signaling
Research Sub Category Developmental Signaling
Quality
Evaluated by Western Blotting in rat brain tissue lysate.
Western Blotting Analysis: 2 μg/mL of this antibody detected LASS5 in rat brain tissue lysate.
Target description
~45 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physical form
Antigen Affinity Purified
Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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