ABS13

Anti-TRABID Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): Ubiquitin thioesterase ZRANB1, TRAF-binding domain-containing protein, hTrabid, Zinc finger Ran-binding domain-containing protein 1, ZRANB1
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human
• technique(s): immunoprecipitation (IP): suitable; western blot: suitable
• NCBI accession no.: NP_060050
• UniProt accession no.: Q9UGI0
• shipped in: wet ice
• Gene Information: human ... ZRANB1(54764)

Description

General description

TRAF-binding domain-containing protein (TRABID) is also known as Ubiquitin thioesterase ZRANB1 and Zinc finger Ran-binding domain-containing protein 1. TRABID specifically hydrolyzes ′Lys-29′-linked and ′Lys-33′-linked diubiquitin, as well as′Lys-63′-linked chains, but with less efficiency. TRABID positively regulates the Wnt signaling pathway, is involved in regulation of cell morphology, cytoskeletal organization, stress fiber dynamics and cell migration, and may also modulate TNF-alpha signaling.

Immunogen

Recombinant protein corresponding to the N-terminus of human TRABID.

Application

Detect TRAF-binding domain-containing protein using this rabbit polyclonal antibody, Anti-TRABID Antibody validated for use in western blotting & IP.

Immunoprecipitation Analysis: 59 µL from a representative lot immunoprecipitated TRABID from 500 µg of HeLa cell lysate. Immunopreciptated protein was then probed with a 1:170 dilution of the same antibody in WB.

Research Category
Metabolism

Research Sub Category
General Post-translation Modification

Quality

Evaluated by Western Blotting in untransfected and TRABID transfected HEK293 cell lysate.

Western Blotting Anlaysis: A 1:1,000 dilution from a representative lot detected TRABID in TRABID transfected HEK293 cell lysate.

Target description

~80 kDa observed

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10-13 - German Storage Class 10 to 13