ABS1025

Anti-NUB1 Antibody

serum, from rabbit

• Synonym(s): NEDD8 ultimate buster 1, Negative regulator of ubiquitin-like proteins 1, Renal carcinoma antigen NY-REN-18
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• antibody form: serum
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: human
• technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• GenBank accession no.: AAD42865
• UniProt accession no.: Q9Y5A7
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... NUB1(51667)

Description

General description

NUB1 is a critical control protein for the ubiquitin proteasome system in that it regulates NEDD8 activity by specifically targeting NEDD8, ubiquitin D (FAT10), and their conjugates to the proteasome for degradation. There are two isoforms, isoform one, (NUB1L) is more efficient at NEDD8 recruitment than isoform 2. Interestingly NUB1 is localized primarily in the nucleus of resting cells and its expression is wide spread from leukocytes in the blood to liver cells and even skeletal muscle cells. Also interesting is that NUB1 was identified early as an auto-antigen in renal carcinomas. NUB1 appears to operate via binding to Asn-51 on NEDD8 which complexes with the UFD1-NPL4 complex that facilitates degradation via the proteasome.

Immunogen

KLH-conjugated linear peptide corresponding to the C-terminus of human NUB1.

Application

Anti-NUB1 Antibody | ABS1025 is an antibody against NUB1 Antibody for use in Western Blotting, Immunohistochemistry (Paraffin).

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected NUB1 in human retina tissue.
Western Blotting Analysis: A representative lot detected NUB1 in NUB1-transfected HEK293 lysate (Schmidtke, G., et al. (2006). J. Biol. Chem. 281:20045-20054).
Western Blotting Analysis: A representative lot detected NUB1 in retinal extracts, Y79, SH-SY5Y, and Hela lysate (Van der Spuy, J., et al. (2003). IOVS. 44(12):5396-5403).
Western Blotting Analysis: A representative lot detected NUB1 in SK-N-SH cell transfected with NUB1-FLAG lysate (Bett, J.S., et al. (2012). PLoS ONE. 7(2):e30866).
Immunohistochemistry Analysis: A representative lot detected NUB1 in retina, cornea, sclera, choroid, iris, and cillary body of developing human eye tissue (Van der Spuy, J., et al. (2003). IOVS. 44(12):5396-5403).

Quality

Evaluated by Western Blotting in SH-SY5Y cell lysate.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected NUB1 in 10 µg of SH-SY5Y cell lysate.

Target description

~70 kDa observed. Uncharacterized band(s) may appear in some lysates.

Other Notes

Concentration: Please refer to lot specific datasheet.

Legal Information

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Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1