ABS1001

Anti-SMO homolog

from rabbit

• Synonym(s): SMO, Smoothened homolog
• UNSPSC Code: 12352203
• eCl@ss: 32160702

Properties

• biological source: rabbit
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: mouse
• technique(s): immunofluorescence: suitable; western blot: suitable
• NCBI accession no.: NP_795970
• UniProt accession no.: P56726
• shipped in: ambient
• Gene Information: mouse ... Smo(319757)

Description

General description

Smoothened homolog (UniProt: P56726; also known as SMO) is encoded by the Smo (also known as Smoh) gene (Gene ID: 319757) in mouse. SMO is a G protein-coupled receptor that associates with the patched protein (PTCH) to transduce the hedgehog′s proteins signaling. In the absence of sonic hedgehog (Shh), PTCH interacts with SMO and inhibits its activity. Shh binding to PTCH removes the inhibitory effect on SMO and allows Gli transcription to proceed. Shh signaling is required throughout embryonic development and is involved in the determination of cell fate and embryonic patterning during early vertebrate development. Disruption of Shh signaling can lead to holoprosencephaly (lack of development of forebrain in the embryo). Abnormal activation of the Shh pathway is seen in a variety of human cancers and in cancer stem cells. Loss of Smo has been linked to impaired hematopoietic stem cell renewal and diminution in the induction of chronic myelogenous leukemia (CML) by Bcr-Abl. On the other hand, constitutively active Smo is shown to cause higher proliferation of CML stem cells.

Specificity

This polyclonal antibody detects smoothened homolog in mouse embryonic fibroblasts.

Immunogen

Epitope: C-terminus

His-tagged recombinant fragment corresponding to 244 amino acids from the C-terminal half of mouse Smoothened homolog.

Application

Detect Smoothened homolog using this rabbit polyclonal Anti-SMO homolog, Cat. No. ABS1001, validated for use in Immunofluorescence and Western Blotting.

Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected Smoothened homolog in Wild Type MEFs (Courtesy of Dr. Pao-Tien Chuang from the University of California, San Francisco).

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Smoothened homolog in wt MEF/ SMO KO MEF (Courtesy of Dr. Pao-Tien Chuang from the University of California, San Francisco).

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Smoothened homolog in wt MEF/ SMO KO MEF (Courtesy of Dr. Pao-Tien Chuang from the University of California, San Francisco).

Immunofluorescence Analysis: A representative lot detected Smoothened homolog in Wild Type MEFS (Chen, M.H., et. al. (2009). Genes Dev. 23(16):1910-28).

Research Category
Signaling

Quality

Evaluated by Western Blotting in wild type mouse embryonic fibroblasts (MEF)/SMO KO MEF.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Smoothened homolog of wild type mouse embryonic fibroblasts (MEF)/SMO KO MEF.

Target description

~81 kDa observed; 87.45 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Affinity Purified

Format: Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable