Kinesin heavy chain isoform 5A (KIF5) is a member of the kinesin-like protein family. These proteins are microtubule-dependent motors that slide along microtubules and transport cellular organelles and mRNA to different parts of the cell. In neurons, KIF5A performs this role in an anterograde fashion from the neuronal cell body to axonal or dendritic terminals. KIF5A was initially differentiated from the ubiquitously expressed kinesin as being highly enriched in subsets of neurons and selectively concentrated in the cell body. Mutations in one of the isoforms of KIF5 (KIF5A) is thought to be involved in hereditary spastic paraplegias.
Specificity
This antibody can recognize isoforms KIF5A, KIF5B, and KIF5C.
Immunogen
KLH-conjugated linear peptide corresponding to human KIF5A.
Application
Detect the KIF5A protein using this Anti-KIF5A validated for use in WB & ICC.
Immunocytochemistry Analysis: 5 µg/mL from a representative lot detected KIF5A in K562 cells.
Research Category Neuroscience
Research Sub Category Developmental Neuroscience
Quality
Evaluated by Western Blot in mouse brain nuclear extract.
Western Blot Analysis: 1 µg/mL of this antibody detected KIF5A in 15 µg/mL of mouse brain nuclear extract.
Target description
~117 kDa observed. Uncharacterized bands may appear at ~45 kDa and ~57 kDa in some lysates.
Physical form
Antigen Affinity Purified
Purified rabbit polyclonal in buffer containing PBS with 0.02% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control Mouse brain nuclear extract
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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