ABN1660

Anti-S-Opsin

from rabbit

• Synonym(s): Short-wave-sensitive opsin 1, Blue cone photoreceptor pigment, Blue-sensitive opsin, BOP, Short wavelength-sensitive cone opsin
• UNSPSC Code: 12352203
• eCl@ss: 32160702

Properties

• biological source: rabbit
• antibody form: unpurified
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: mouse
• species reactivity (predicted by homology): rat (based on 100% sequence homology)
• technique(s): electron microscopy: suitable; immunofluorescence: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• isotype: IgG
• NCBI accession no.: NP_031564
• UniProt accession no.: P51491
• shipped in: ambient
• target post-translational modification: unmodified
• Gene Information: mouse ... Opn1Sw(12057)

Description

General description

Short-wave-sensitive opsin 1 (UniProt: P51491; also known as S opsin, Blue cone photoreceptor pigment, Blue-sensitive opsin, BOP, Short wavelength-sensitive cone opsin) is encoded by the Opn1sw (also known as Bcp) gene (Gene ID: 12057) in murine species. Opsins are G-protein coupled protein with photoreceptor activity. Opsins contain seven transmembrane alpha-helical domains connected by three extra-cellular and three cytoplasmic loops. They are light-sensitive proteins found in photoreceptor cells of the retina. Based on sequence homology, the opsins are classified into six subfamilies: the vertebrate opsin/encephalopsin subfamily, the Go opsin subfamily, the Gs opsin subfamily, the invertebrate Gq opsin subfamily, the photoisomerase subfamily, and the neuropsin subfamily. They share less than 20 percent identity between subfamilies. Opsins provide the necessary environment for the absorption of light at a particular wavelength. Therefore, by providing a different opsin to the retinal, organisms can sense light of different wavelengths. S-opsin is a multi-pass membrane protein that is expressed in cone photoreceptor cells. They are light-absorbing molecules that mediate vision and contain an apoprotein, opsin, which is covalently linked to cis-retinal. It can increase spectral sensitivity in dim light.

Specificity

This polyclonal antibody specifically detects short-wave-sensitive opsin 1 in murine species. Murine and human S-opsin display 88% sequence homology. It targets an epitope within 17 amino acids from the C-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 17 amino acids from the C-terminal end of murine short-wave-sensitive opsin 1.

Application

Anti-S-Opsin, Cat. No. ABN1660, is a highly specific rabbit polyclonal antibody that targets Short-wave-sensitive opsin 1 and has been tested in Electron Microscopy, Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.

Electron Microscopy Analysis: A representative lot detected S-Opsin in P30 retinas from COP-T/WT and MOP-T/WT mice (Chakraborty, D., et. al. (2009). Hum Mol Genet. 18(5):797-808).

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected S-Opsin in mouse retinal extract (Courtesy of Dr. Muna Naash at University of Houston, Houston, TX).

Immunofluorescence Analysis: A representative lot detected S-Opsin in paraffin-embedded retinal and outer segment disc sections from WT and COP-T/WT mice (Chakraborty, D., et. al. (2009). Hum Mol Genet. 18(5):797-808).

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected S-Opsin in mouse retina tissue.

Quality

Evaluated by Western Blotting in NRL-/-KO Ms retina lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected S-Opsin in 5 µg of NRL-/-KO mouse retina lysate.

Target description

~ 37 kDa observed; 38.92 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Unpurified

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable