ABN1444

Anti-IMPACT

from rabbit, purified by affinity chromatography

• Synonym(s): Protein IMPACT, Imprinted and ancient gene protein homolog
• UNSPSC Code: 12352203
• eCl@ss: 32160702

Properties

• biological source: rabbit
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human, mouse
• species reactivity (predicted by homology): nonhuman primates (based on 100% sequence homology)
• technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• NCBI accession no.: NP_060909
• UniProt accession no.: Q9P2X3
• shipped in: ambient
• target post-translational modification: unmodified
• Gene Information: human ... IMPACT(55364)

Description

General description

Protein IMPACT (UniProt Q9P2X3; also known as Imprinted and ancient gene protein homolog) is encoded by the IMPACT gene (Gene ID 55364) in human. IMPACT is a cytoplasmic protein preferentially expressed in neurons. IMPACT is highly abundant in the hypothalamus and in scattered neurons in other areas, such as the hippocampal interneurons. IMPACT and its yeast ortholog Yih1 contain an RWD domain (a.a. 14-116 of human and mouse IMPACT) that interacts with GCN1 and competes against GCN2 for GCN1 binding. IMPACT and Yih1 overexpression in mouse embryonic fibroblasts (MEFs) and yeasts inhibits GCN2 activation induction. IMPACT is a developmentally regulated protein that promotes neuritogenesis, while GCN2 strongly inhibits spontaneous neurite outgrowth. Increased abundance of IMPACT in differentiated neuronal cells enhances translation initiation by preventing GCN2 signaling.

Specificity

This polyclonal antibody was raised against the C-terminal end sequence present in both spliced isoforms of human IMPACT reported by UniProt (Q9P2X3).

Immunogen

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to the C-terminal sequence of human IMPACT.

Application

Anti-IMPACT Antibody, Cat. No. ABN1444, is a highly specific rabbit polyclonal antibody that targets IMPACT and has been tested in Immunohistochemistry and Western Blotting.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected IMPACT in human prostate cancer, as well as in normal human cerebral cortex and mouse cerebellum tissue sections.

Research Category
Neuroscience

Quality

Evaluated by Western Blotting in human testis tissue lysate.

Western Blotting Analysis: A 1:1000 dilution of this antibody detected IMPACT in 10 µg of human testis tissue lysate.

Target description

~36 kDa observed. 36.48/23.00 kDa (human isoform 1/2) and 36.28 kDa (mouse) calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Affinity purified.

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4) 150 mM NaCl with 0.05% sodium azide

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected IMPACT in human prostate cancer

as well as in normal human cerebral cortex and mouse cerebellum tissue sections.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable