ABN1029

Anti-CHD-8 Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): Chromodomain-helicase-DNA-binding protein 8, CHD-8, ATP-dependent helicase CHD8, Axis duplication inhibitor, Duplin
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human
• species reactivity (predicted by homology): rat (based on 100% sequence homology)
• technique(s): immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• NCBI accession no.: NP_075222
• UniProt accession no.: Q9JIX5
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... CHD8(57680)

Description

General description

CHD-8 is a DNA helicase that acts as a chromatin remodeling factor as well as a transcriptional regulator. CHD-8 acts as a transcription repressor by remodeling chromatin structure and recruiting histone H1 to various genes such as p53. CHD-8 regulates beta catenin activity by suppressing the promoter activity of several beta catenin targeted genes. CHD-8 also suppresses STAT3 transcriptional activation in a similar fashion. CHD-8 is a component of multiple chromatin complexes and localizes to the nucleus and active chromatin. Interestingly mutations in CHD-8 are associated with a form of autism, Autism 18 as well as some forms of developmental delay and cognitive impairment, demonstrating CHD-8’s potential importance in developmental brain function, and behavior.

Immunogen

Recombinant protein corresponding to rat CHD-8.

Application

Anti-CHD-8 | ABN1029 is an antibody against CHD-8 for use in Western Blotting, Immunocytochemistry, Immunoprecipitation.

Research Category
Neuroscience

Research Sub Category
Developmental Signaling

Western Blotting Analysis: A representative lot detected CHD-8 in A431 cell lysate (Sakamoto, I., et al. (2000). JBC. 275:32871-32878).
Immunoprecipitation Analysis: A representative lot immunoprecipitated CHD-8 in β-catenin cell lysate (Sakamoto, I., et al. (2000). JBC. 275:32871-32878).
Immunocytochemistry Analysis: A representative lot detected CHD-8 in Lcells and SW480 cells (Sakamoto, I., et al. (2000). JBC. 275:32871-32878).

Quality

Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected CHD-8 in 10 µg of HepG2 cell lysate.

Target description

~290 kDa observed. Uncharacterized band(s) may appear in some lysates.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2