ABE577
Anti-Myb Binding Protein 1A Antibody
serum, from rabbit
Synonym(s):
Myb-binding protein 1A
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
rabbit
Quality Level
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human
technique(s)
ChIP: suitable
immunocytochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... MYBBP1A(10514)
General description
Myb-binding protein 1A (MYBBP1A) interacts with sequence specific DNA-binding proteins in order to activate or repress transcription. Transcriptional repression via Myb-binding protein 1A is thought to be mediated by histone deacetylase activity (HDAC activity).
Specificity
This antibody detects the full length and cleaved forms of Myb Binding Protein 1A.
Immunogen
GST-tagged recombinant protein corresponding to human Myb Binding Protein 1A.
Application
Anti-Myb Binding Protein 1A Antibody is a highly specific rabbit polyclonal antibody, that targets Myb-binding protein 1A & has been tested in western blotting & ICC.
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Myb Binding Protein 1A in diploid human lung embryonic fibroblasts (Prof. Atilla Nemeth, University of Regensburg, Germany.).
Immunocytochemistry Analysis: A representative lot from an independent laboratory detected Myb Binding Protein 1A in HeLa cells (Hochstatter, J., et al. (2012). J Biol Chem. 287(29):24365-24377.).
Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot from an independent laboratory immunoprecipitated Myb Binding Protein 1A in ChIP (Hochstatter, J., et al. (2012). J Biol Chem. 287(29):24365-24377.).
Immunocytochemistry Analysis: A representative lot from an independent laboratory detected Myb Binding Protein 1A in HeLa cells (Hochstatter, J., et al. (2012). J Biol Chem. 287(29):24365-24377.).
Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot from an independent laboratory immunoprecipitated Myb Binding Protein 1A in ChIP (Hochstatter, J., et al. (2012). J Biol Chem. 287(29):24365-24377.).
Quality
Evaluated by Western Blotting in A431 cell lysate.
Western Blotting Analyis: A 1:1,000 dilution from a representative lot detected Myb Binding Protein 1A in 10 µg of A431 cell lysate.
Western Blotting Analyis: A 1:1,000 dilution from a representative lot detected Myb Binding Protein 1A in 10 µg of A431 cell lysate.
Target description
~160 kDa observed. Uncharacterized band(s) may be observed in some cell lysates. The full length of this protein is observed at ~160 kDa; however, it is proteolytically cleaved into ~140 kDa and ~67 kDa fragments (Hochstatter, J., et al. (2012). J Biol Chem. 287(29):24365-24377.; Mori, S., et al. (2012). PLoS One. 7(10):e39723.).
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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