ABE500

Anti-WBP-7 Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): Histone-lysine N-methyltransferase MLL4, Lysine N-methyltransferase 2B, KMT2B, Myeloid/lymphoid or mixed-lineage leukemia protein 4, Trithorax homolog 2, WW domain-binding protein 7, WBP-7
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: rat, human, mouse
• technique(s): western blot: suitable
• NCBI accession no.: NP_055542
• UniProt accession no.: Q9UMN6
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... KMT2B(9757)

Description

General description

WW domain-binding protein 7 (WBP-7) is also called Histone-lysine N-methyltransferase MLL4, Lysine N-methyltransferase 2B (KMT2B), Myeloid/lymphoid or mixed-lineage leukemia protein 4 and Trithorax homolog 2. WBP-7 is a histone methyltransferase that methylates ′Lys-4′ of histone H3, a specific tag for epigenetic transcriptional activation. WBP-7 is important in beta-globin locus transcription regulation and for controlling bulk H3K4 trimethylation (H3K4me3), global transcriptional silencing, oocyte survival and normal zygotic genome activation during oocyte growth and development. WBP-7 is widely expressed with the highest levels in testis. WBP-7 is also detected in brain, bone marrow, heart, muscle, kidney, placenta, spleen, thymus, prostate, ovary, intestine, colon, peripheral blood lymphocytes and pancreas and is found to often be amplified in pancreatic carcinomas.

Specificity

This antibody recognizes WBP-7 near the C-Terminus.

Immunogen

Epitope: Near C-Terminus

KLH-conjugated linear peptide corresponding to human WBP-7 near the C-Terminus.

Application

Detect WW domain-binding protein 7 using this rabbit polyclonal antibody, Anti-WBP-7 Antibody validated for use in western blotting.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

Quality

Evaluated by Western Blotting in MCF7 cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected MCF7 in 10 µg of MCF7 cell lysate.

Target description

~75 kDa observed. The full length form and the cleaved form of WBP-7 may be observed at 300 kDa and 75 kDa, respectively (Natarajam, T. G., et al. (2010). Cancer Cell Int. 10:13).

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
MCF7 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable