ABE499

Anti-JMJD2D Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): Lysine-specific demethylase 4D, JmjC domain-containing histone demethylation protein 3D, Jumonji domain-containing protein 2D
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human
• species reactivity (predicted by homology): chimpanzee (based on 100% sequence homology)
• technique(s): immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• NCBI accession no.: NP_060509
• UniProt accession no.: Q6B0I6
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... KDM4D(55693)

Description

General description

Jumonji domain-containing protein 2D (JMJD2D) is also called Lysine-specific demethylase 4D and JmjC domain-containing histone demethylation protein 3D. JMJD2D is a histone demethylase that specifically demethylates both di- and trimethylated H3 ′Lys-9′, but not monomethylated histone H3 ′Lys-9′ residues. JMJD2D also does not demethylate histone H3 ′Lys-4′, H3 ′Lys-27′, H3 ′Lys-36′ or H4 ′Lys-20′.

Specificity

Other homologies: Bovine, Porcine, Canine, and Rhesus Macaque (93% sequence homology), Mouse (87% sequence homology), Rat (80% sequence homology).

Immunogen

KLH-conjugated linear peptide corresponding to human JMJD2D.

Application

Detect Jumonji domain-containing protein (JMJD) using this rabbit polyclonal antibody, Anti-JMJD2D Antibody validated for use in western blotting, IHC & IP.

Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected JMJD2D in human testis and liver tissues.

Immunoprecipitation Analyis: 20 µg from a representative lot detected JMJD2D in 500 µg of HeLa cell lysate.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected JMJD2D in 10 µg of HeLa cell lysate

Target description

~58 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HeLa cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable