ABE484

Anti-LOXL2

from rabbit, purified by affinity chromatography

• Synonym(s): Lysyl oxidase homolog 2, EC: 1.4.3.13, Lysyl oxidase-like protein 2, Lysyl oxidase-related protein 2, Lysyl oxidase-related protein WS9-14
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human
• species reactivity (predicted by homology): mouse (based on 100% sequence homology), rat (based on 100% sequence homology), bovine (based on 100% sequence homology)
• packaging: antibody small pack of 25 μg
• technique(s): immunohistochemistry: suitable (paraffin); immunoprecipitation (IP): suitable; western blot: suitable
• NCBI accession no.: NP_002309.1
• UniProt accession no.: Q9Y4K0
• shipped in: ambient
• target post-translational modification: unmodified
• Gene Information: human ... LOXL2(4017) ; mouse ... Loxl2(94352)

Description

General description

Lysyl oxidase homolog 2 (UniProt: Q9Y4K0; also known as EC: 1.4.3.13, Lysyl oxidase-like protein 2, Lysyl oxidase-related protein 2, Lysyl oxidase-related protein WS9-1, LOXL2) is encoded by the LOXL2 gene (Gene ID: 4017) in human. LOXL2 localizes to both cytoplasmic and nuclear region. It catalyzes the post-translational oxidative deamination of lysine residues on target proteins leading to the formation of deaminated lysine (allysine). When secreted in extracellular matrix, it promotes cross-linking of extracellular matrix proteins by mediating oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. LOXL2 also acts as a regulator of sprouting angiogenesis, probably via collagen IV scaffolding. When present in the nucleus, it acts as a transcription corepressor and specifically mediates deamination of trimethylated Lysine 4 of histone H3 (H3K4me3), a specific tag for epigenetic transcriptional activation. Overexpression of LOXL2 has been reported in a number of cancers and its ability to promote epithelial to mesenchymal transition suggest that it might play a role in tumor progression. Higher expression has been correlated with metastasis and reduced survival in patients with aggressive breast cancer. LOXL2 activity is strongly induced in hypoxia and it is reported to be a direct transcriptional target of HIF1A.

Lysyl oxidase homolog 2 (UniProt: Q9Y4K0; also known as EC: 1.4.3.13, Lysyl oxidase-like protein 2, Lysyl oxidase-related protein 2, Lysyl oxidase-related protein WS9-14, LOXL2) is encoded by the LOXL2 gene (Gene ID: 4017) in human. LOXL2 localizes to both cytoplasmic and nuclear region. It catalyzes the post-translational oxidative deamination of lysine residues on target proteins leading to the formation of deaminated lysine (allysine). When secreted in extracellular matrix, it promotes cross-linking of extracellular matrix proteins by mediating oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. LOXL2 also acts as a regulator of sprouting angiogenesis, probably via collagen IV scaffolding. When present in the nucleus, it acts as a transcription corepressor and specifically mediates deamination of trimethylated Lysine 4 of histone H3 (H3K4me3), a specific tag for epigenetic transcriptional activation. Overexpression of LOXL2 has been reported in a number of cancers and its ability to promote epithelial to mesenchymal transition suggest that it might play a role in tumor progression. Higher expression has been correlated with metastasis and reduced survival in patients with aggressive breast cancer. LOXL2 activity is strongly induced in hypoxia and it is reported to be a direct transcriptional target of HIF1A.

Specificity

This rabbit polyclonal antibody detects Lysyl oxidase homolog 2 in human cells. It targets an epitope within 16 amino acids from the N-terminal half.

Immunogen

Epitope: N-terminus

KLH-conjugated linear peptide corresponding to 16 amino acids from the N-terminal half of human Lysyl oxidase homolog 2.

Application

Anti-LOXL2, Cat. No. ABE484, is a highly specific rabbit polyclonal antibody that targets Lysyl oxidase homolog 2 and has been tested in Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

Research Category
Epigenetics & Nuclear Function

Western Blotting Analysis: A 1:500 dilution from a representative lot detected LOXL2 in MDA-MB-231 cell lysate.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected LOXL2 in human prostate and human placenta tissues.
Immunoprecipitation Analysis: A representative lot detected LOXL2 in LoxL2 expressing vector (Courtesy Dr Sandra Peiro from the Institut Hospital del Mar d′Investigacions Mèdiques (IMIM) in Barcelona Spain ).

Western Blotting Analysis: A 1:500 dilution from a representative lot detected LOXL2 in MDA-MB-231 cell lysate.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected LOXL2 in human prostate and human placenta tissues.

Immunoprecipitation Analysis: A representative lot detected LOXL2 in LoxL2 expressing vector (Courtesy Dr Sandra Peiro from the Institut Hospital del Mar d′Investigacions Mèdiques (IMIM) in Barcelona Spain).

nti-LOXL2, Cat. No. ABE484, is a highly specific rabbit polyclonal antibody that targets Lysyl oxidase homolog 2 and has been tested in Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

Quality

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected LOXL2 in A431 cell lysate.

Target description

~82 kDa obseerved; 86.72 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Affinity Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable