ABE323

Anti-dimethyl Histone H4 Antibody (Arg17)

from rabbit, purified by affinity chromatography

• Synonym(s): Histone H4
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human, mouse
• technique(s): dot blot: suitable; immunocytochemistry: suitable; western blot: suitable
• NCBI accession no.: NP_001029249
• UniProt accession no.: P62805
• shipped in: wet ice
• target post-translational modification: dimethylation (Arg17)
• Gene Information: human ... HIST2H4B(554313)

Description

General description

Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. Acetylation of histone H4 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).

Specificity

This antibody recognizes the N-terminus of asymmetric Histone H4 dimethylated at Arg17.

Immunogen

KLH-conjugated linear peptide corresponding to the N-terminus of human asymmetric Histone H4 dimethylated at Arg17.

Application

Detect Histone H4 using this rabbit polyclonal antibody, Anti-dimethyl Histone H4 Antibody (Arg17) validated for use in western blotting, Dot Blot & ICC.

Dot Blot (Specificity) Analysis: A 1:200 dilution from a representative lot detected asymmetric Histone H4 dimethylated at Arg17, but not unmethylated Histone H4 or other peptides corresponding to modified histones.

Immunocytochemistry Analysis: A 1:2,000 dilution from a representative lot detected Histone H4 dimethylated at Arg17 in MEF-1 cells.

Alexa Fluor^™ is a registered trademark of Life Technologies.

Quality

Evaluated by Western Blotting in untreated and CARM1 treated Histone H4 recombinant proteins.

Western Blotting Analysis: 0.25 µg/mL of this antibody detected asymmetric Histone H4 dimethylated at Arg17 in CARM1 treated Histone H4 recombinant proteins.

Target description

~13 kDa observed

Analysis Note

Control
Untreated and CARM1 treated Histone H4 recombinant proteins.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable