ABE2910
Anti-DNMT3L
from rabbit
Synonym(s):
DNA (cytosine-5)-methyltransferase 3-like
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
rabbit
Quality Level
antibody form
unpurified
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse
packaging
antibody small pack of 25 μL
technique(s)
immunocytochemistry: suitable
immunofluorescence: suitable
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Gene Information
mouse ... Dnmt3L(54427)
General description
DNA (cytosine-5)-methyltransferase 3-like (UniProt: Q9CWR8; also known as DNMT3L) is encoded by the Dnmt3l gene (Gene ID: 54427) in murine species. DNMT3L is a catalytically inactive regulatory factor of DNA methyltransferases that can either promote or inhibit DNA methylation depending on the context.
It is shown to be essential for the function of DNMT3A and DNMT3B. It activates DNMT3A and DNMT3B by binding to their catalytic domain. It acts by accelerating the binding of DNA and S-adenosyl-L-methionine (AdoMet) to the methyltransferases and dissociates from the complex after DNA binding to the methyltransferases. DNMT3L recognizes unmethylated histone H3 lysine 4 (H3K4me0) and induces de novo DNA methylation by recruitment or activation of DNMT3. In germ cells, it is required for the methylation of imprinted loci together with DNMT3A. In male germ it is essential for the methylation of retrotransposons, which prevents their mobilization. DNMT3A also plays a key role in embryonic stem cells (ESCs) by acting both as a positive and negative regulator of DNA methylation. It promotes DNA methylation of housekeeping genes together with DNMT3A and DNMT3B. It can also serve as an inhibitor of DNA methylation at the promoter of bivalent genes. It is reported to promote differentiation of ESCs into primordial germ cells by inhibiting DNA methylation at the promoter of RHOX5, thereby activating its expression. In testicular cells it is first observed in non-dividing prospermatogonia after 12.5 dpc and its level reaches its highest at about the time of birth and then rapidly declines and is non-detectable six days postpartum when most of the prospermatognia are differentiated into dividing spermatogonial stem cells.
It is shown to be essential for the function of DNMT3A and DNMT3B. It activates DNMT3A and DNMT3B by binding to their catalytic domain. It acts by accelerating the binding of DNA and S-adenosyl-L-methionine (AdoMet) to the methyltransferases and dissociates from the complex after DNA binding to the methyltransferases. DNMT3L recognizes unmethylated histone H3 lysine 4 (H3K4me0) and induces de novo DNA methylation by recruitment or activation of DNMT3. In germ cells, it is required for the methylation of imprinted loci together with DNMT3A. In male germ it is essential for the methylation of retrotransposons, which prevents their mobilization. DNMT3A also plays a key role in embryonic stem cells (ESCs) by acting both as a positive and negative regulator of DNA methylation. It promotes DNA methylation of housekeeping genes together with DNMT3A and DNMT3B. It can also serve as an inhibitor of DNA methylation at the promoter of bivalent genes. It is reported to promote differentiation of ESCs into primordial germ cells by inhibiting DNA methylation at the promoter of RHOX5, thereby activating its expression. In testicular cells it is first observed in non-dividing prospermatogonia after 12.5 dpc and its level reaches its highest at about the time of birth and then rapidly declines and is non-detectable six days postpartum when most of the prospermatognia are differentiated into dividing spermatogonial stem cells.
Specificity
This rabbit polyclonal antibody detects Dnmt3l in murine cells.
Immunogen
Epitope: unknown
His-tagged full length (421 aa) murine recombinant DNA (cytosine-5)-methyltransferase 3-like (DNMT3L).
Application
Anti-DNMT3L, Cat. No. ABE2910, is a highly specific rabbit polyclonal antibody that targets DNA (cytosine-5)-methyltransferase 3-like and has been tested in Immunocytochemistry, Immunofluorescence, and Western Blotting.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Western Blotting Analysis: A representative lot detected Dnmt3l in undifferentiated J1 mouse embryonic stem cells (Nimura, K., et. al. (2006). Genes Cells. 11(10):1225-37).
Immunofluorescence Analysis: A representative lot detected Dnmt3l in mouse testes (Nimura, K., et. al. (2006). Genes Cells. 11(10):1225-37) and XY germ cells at E15.5 (Kato, Y., et. al. (2016). Nat Commun. 7:11272).
Immunocytochemistry Analysis: A representative lot detected Dnmt3l in undifferentiated J1 mouse embryonic stem cells (Nimura, K., et. al. (2006). Genes Cells. 11(10):1225-37).
Immunofluorescence Analysis: A representative lot detected Dnmt3l in mouse testes (Nimura, K., et. al. (2006). Genes Cells. 11(10):1225-37) and XY germ cells at E15.5 (Kato, Y., et. al. (2016). Nat Commun. 7:11272).
Immunocytochemistry Analysis: A representative lot detected Dnmt3l in undifferentiated J1 mouse embryonic stem cells (Nimura, K., et. al. (2006). Genes Cells. 11(10):1225-37).
Quality
Evaluated by Western Blotting in mouse embryonic stem cell lysate.
Western Blotting Analysis: A 1:2,000 dilution of this antibody detected Dnmt3l in 10 µg of mouse embryonic stem cell lysate.
Western Blotting Analysis: A 1:2,000 dilution of this antibody detected Dnmt3l in 10 µg of mouse embryonic stem cell lysate.
Target description
~50 kDa observed; 47.99 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Format: Unpurified
Rabbit polyclonal antiserum with 0.05% sodium azide.
Unpurified
Storage and Stability
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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