ABE281
Anti-EZH1 Antibody
from rabbit, purified by affinity chromatography
Synonym(s):
Histone-lysine N-methyltransferase EZH1, ENX-2, Enhancer of zeste homolog 1
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human, mouse
species reactivity (predicted by homology)
rat (based on 100% sequence homology), bovine (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology)
technique(s)
immunohistochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... EZH1(2145)
General description
Polycomb protein group complexes are responsible for the regulation of hundreds of genes in mammals and insects. These proteins are responsible for the assembly and packaging of chromatin and for the induction of methylation on Histone H3. EZH1 is the catalytic subunit of the PRC2/EED-EZH1 complex. This complex methylates Lys-27 of histone H3, which leads to transcriptional repression of the affected target gene. EZH1 is required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in maintaining the identity of embryonic stem cells.
Specificity
Other homologies: Porcine (94% sequence homology).
This antibody potentially recognizes all isoforms of EZH1.
Immunogen
KLH-conjugated linear peptide corresponding to human EZH1.
Application
Detect the EZH1 protein using this Anti-EZH1 validated for use in IHC & WB.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected EZH1 in human lung and kidney tissues.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histone Modifying Proteins
Histone Modifying Proteins
Quality
Evaluated by Western Blot in mouse embryonic stem cell lysate.
Western Blot Analysis: 0.2 µg/mL of this antibody detected EZH1 in 10 µg of mouse embryonic stem cell lysate.
Western Blot Analysis: 0.2 µg/mL of this antibody detected EZH1 in 10 µg of mouse embryonic stem cell lysate.
Target description
~85 kDa observed. Uniprot states 5 isoforms for EZH1. This antibody potentially recognizes all isoforms. Isoform A is 85,271 Daltons. Isoform B is 86,025 Daltons. Isoform C is 80,699 Daltons. Isoform D is 76,952 Daltons. Isoform E is 68,961 Daltons. (Margueron, 2008).
Linkage
Replaces: 04-1047
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Mouse embryonic stem cell lysate
Mouse embryonic stem cell lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Sebastian C B Bremer et al.
Digestion, 102(2), 227-235 (2019-11-07)
Colorectal cancer (CRC) is the leading gastrointestinal malignancy. The development from premalignant intraepithelial lesions leading to invasive cancer is paradigmatic for the stepwise carcinogenesis of epithelial cancers, but the knowledge of the underlying mechanism of carcinogenesis and progression of CRC
Jing Liang et al.
British journal of pharmacology, 176(17), 3206-3219 (2019-06-05)
The increased proliferation and migration of vascular smooth muscle cells (VSMCs) after arterial injury contributes greatly to the pathogenesis of neointimal hyperplasia. As a major component of epigenetics, histone methylation plays an important role in several cardiovascular diseases. However, its
Sebastian C B Bremer et al.
Cancers, 14(12) (2022-06-25)
Tumor grading is a robust prognostic predictor in patients with neuroendocrine neoplasms (NEN) and guides therapy, especially in tumors with high proliferation. NEN can be separated into well-differentiated and poorly differentiated types. The more aggressive NEN have been further separated
Jiajia Wang et al.
Science advances, 6(33), eaaz6477-eaaz6477 (2020-08-28)
Mutations in the polycomb repressive complex 2 (PRC2) can cause Weaver-like syndrome, wherein a patient cohort exhibits abnormal white matter; however, PRC2 functions in CNS myelination and regeneration remain elusive. We show here that H3K27me3, the PRC2 catalytic product, increases
Xue-Wei Wang et al.
The Journal of clinical investigation, 134(3) (2023-11-28)
Current treatments for neurodegenerative diseases and neural injuries face major challenges, primarily due to the diminished regenerative capacity of neurons in the mammalian CNS as they mature. Here, we investigated the role of Ezh2, a histone methyltransferase, in regulating mammalian
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