ABE2600

Anti-DNA Helicase B (HELB)

from rabbit

• Synonym(s): HELB
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: mouse
• packaging: antibody small pack of 25 μL
• technique(s): immunocytochemistry: suitable; western blot: suitable
• isotype: IgG
• NCBI accession no.: NP_536694
• UniProt accession no.: Q6NVF4
• target post-translational modification: unmodified
• Gene Information: mouse ... Helb(117599)

Description

General description

DNA helicase B (UniProt: Q6NVF4; also known as EC: 3.6.4.12, HELB) is encoded by the Helb gene (Gene ID: 117599) in murine species. HELB helicase B is is a predominantly nuclear helicase that can undergo phosphorylation at Serine 942 by CDK2 during the G1/S transition that results in its export into the cytoplasm as cells approach and progress through S phase. Its nuclear export signal lies within amino acids 1022-1046. It serves as a 5 -3′ DNA helicase involved in DNA damage response by acting as an inhibitor of DNA end resection. It is shown to bind to RPA1 and this interaction promotes HELB recruitment to chromatin following DNA damage. Following DNA damage it is recruited to sites of double-strand breaks by the Replication Protein A (RPA) complex. Recruitment to single-stranded DNA (ssDNA) following DNA damage leads to inhibit the nucleases catalyzing resection, such as EXO1, BLM and DNA2, possibly via the 5′-3′ ssDNA translocase activity of HELB. HELB is reported to act independently of TP53BP1. Loss of DNA helicase B results in PARP inhibitor resistance in BRCA1-deficient tumor cells.

Specificity

This rabbit polyclonal antibody detects DNA helicase B in murine species. It targets an epitope within 227 amino acids from the C-terminal region.

Immunogen

Epitope: C-terminus

GST-tagged recombinant fragment corresponding to 227 amino acids from the C-terminal half of murine DNA helicase B.

Application

Anti-DNA Helicase B (HELB) Antibody, Cat. No. ABE2600, is a highly specific rabbit polyclonal antibody that targets DNA helicase B (HELB) and has been tested for use in Immunocytochemistry and Western Blotting.

Immunocytochemistry Analysis: A representative lot detected DNA Helicase B (HELB) in Immunocytochemistry applications (Tkac, J., et. al. (2016). Mol Cell. 61(3):405-418).

Western Blotting Analysis: A representative lot detected DNA Helicase B (HELB) in Western Blotting applications (Tkac, J., et. al. (2016). Mol Cell. 61(3):405-418).

Research Category
Epigenetics & Nuclear Function

Quality

Evaluated by Western Blotting in wild-type mouse embryonic fibroblasts .

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected DNA helicase B (HELB) in lysate from wild-type mouse embryonic fibroblasts (MEF) and MEF heterogygotes for HELB.

Target description

145 kDa observed; 121.47 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Affinity Purified

Format: Purified

Purified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, pH7.4 with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.